Establishment of a novel method to evaluate peritoneal microdissemination and therapeutic effect using luciferase assay.
Establishment of a novel method to evaluate peritoneal microdissemination and therapeutic effect using luciferase assay.
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DOI：
10.1111/cas.12872
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发表时间：
2016-03
期刊：
影响因子：
5.7
通讯作者：
Asao T
中科院分区：
文献类型：
--
作者：
Takahashi R;Yokobori T;Osone K;Tatsuki H;Takada T;Suto T;Yajima R;Kato T;Fujii T;Tsutsumi S;Kuwano H;Asao T
腹膜扩散是腹腔恶性肿瘤患者复发的主要原因。有效的抗癌药物和治疗方案对于改善这些患者的预后是必要的。然而，以前的研究使用小鼠模型的腹膜传播没有检测到任何药物对腹膜微转移的影响。在此，我们使用荧光素酶检测来评估活体动物的腹膜微转移，并建立了一个准确的早期腹膜微转移的小鼠模型来评估肿瘤发生和药物疗效。体内荧光素酶测定的发光强度与体外荧光素酶测定和肠系膜重量评估的肿瘤扩散程度呈正相关。该模型比以前的模型具有优势，因为在没有细胞损伤的情况下验证了最佳的白细胞介素浓度，并且可以定量评估腹膜微播散。因此，它是一个有用的模型，以验证腹膜微转移形成和评价药物疗效，而不杀死小鼠。
Peritoneal dissemination is a major cause of recurrence in patients with malignant tumors in the peritoneal cavity. Effective anticancer agents and treatment protocols are necessary to improve outcomes in these patients. However, previous studies using mouse models of peritoneal dissemination have not detected any drug effect against peritoneal micrometastasis. Here we used the luciferase assay to evaluate peritoneal micrometastasis in living animals and established an accurate mouse model of early peritoneal microdissemination to evaluate tumorigenesis and drug efficacy. There was a positive correlation between luminescence intensity in in vivo luciferase assay and the extent of tumor dissemination evaluated by ex vivo luciferase assay and mesenteric weight. This model has advantages over previous models because optimal luciferin concentration without cell damage was validated and peritoneal microdissemination could be quantitatively evaluated. Therefore, it is a useful model to validate peritoneal micrometastasis formation and to evaluate drug efficacy without killing mice.