STD1 (MSN3) interacts directly with the TATA-binding protein and modulates transcription of the SUC2 gene of Saccharomyces cerevisiae.
STD1 (MSN3) interacts directly with the TATA-binding protein and modulates transcription of the SUC2 gene of Saccharomyces cerevisiae.
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STD1 (MSN3) 直接与 TATA 结合蛋白相互作用并调节酿酒酵母 SUC2 基因的转录。
DOI：
10.1093/nar/23.16.3174
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发表时间：
1995
影响因子：
14.9
通讯作者：
Schmidt,MC
中科院分区：
文献类型：
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作者：
Tillman,TS;Ganster,RW;Jiang,R;Carlson,M;Schmidt,MC
STD1 (MSN3)was Isolated independently as a multicopy suppressor of mutations in the TATA-binding protein and in SNF4, suggesting that STD1 might couple the SNF1 kinase signaling pathway to the transcriptional machinery. We report here a direct physical interaction betweenSTD1and the TATA-binding protein (TBP), observedIn vivoby the two-hybrid system and In vitro by binding studies.STD1bound both native TBP In yeast cell-free extracts and purified recombinant TBP. This interaction was altered when TBPΔ57 was used, suggesting a role for the non-conserved N-terminal domain of TBP in mediating proteinprotein interactions. We also show that perturbation of STD1-TBP stoichiometry altersSUC2expressionIn vivoand that this effect Is dependent on the N-terminal domain of TBP. The activation of SUC2 expression by increased copy number of STD1 occurs at the level of mRNA accumulation and it requires the same TATA element and uses the same transcription start site as does activation of SUC2 by glucose limitation. Taken together, these results suggest that STD1 modulatesSUC2transcription through direct interactions with TBP.
发育过程中的细胞相互作用
DOI：
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发表时间：
1965
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影响因子：
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作者：
M. Abercrombie
通讯作者：
M. Abercrombie
“小鼠髓磷脂碱性蛋白基因的启动子元件在 NG108-15 神经元/神经胶质细胞基因中有效发挥作用。”
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