Sex determination based on fecal DNA analysis of the amelogenin gene in sika deer (Cervus nippon).
Sex determination based on fecal DNA analysis of the amelogenin gene in sika deer (Cervus nippon).
复制标题
基于梅花鹿 (Cervus nippon) 粪便 DNA 釉原蛋白基因分析的性别决定。
DOI：
10.1292/jvms.62.669
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Y. Mori
中科院分区：
文献类型：
--
作者：
K. Yamauchi;S. Hamasaki;K. Miyazaki;T. Kikusui;Y. Takeuchi;Y. Mori
建立了一种利用粪便DNA鉴定西卡鹿性别的方法。我们测定了西卡鹿釉原蛋白基因的部分序列，该基因存在于X和Y染色体上，在Y染色体上有一个缺失区。根据两性二态性序列，设计了一对引物，可以扩增出雌雄间长度不同的DNA片段。从37份鹿粪样品中检测到34份PCR产物，用该方法估测的鹿群性别与实际性别完全吻合。
A sex determination method using DNA extracted from feces has been developed for sika deer (Cervus nippon). We determined a partial sequence of the amelogenin gene of sika deer, which exists on both X and Y chromosomes with a deletion region on the Y chromosome. Based on the sexually dimorphic sequences, we designed a pair of primers which could amplify DNA fragments the lengths of which are different between males and females. PCR products were detected in 34 out of 37 fecal samples collected from captured deer and the sexes estimated by the present method were perfectly matched with the actual sexes.