Cell surface alpha-D-galactopyranosyl end groups: use as markers in the isolation of murine tumor cell lines with different cancer-causing potentials.
Cell surface alpha-D-galactopyranosyl end groups: use as markers in the isolation of murine tumor cell lines with different cancer-causing potentials.
复制标题
细胞表面α-D-吡喃半乳糖基端基：用作分离具有不同致癌潜力的鼠肿瘤细胞系的标记物。
DOI：
10.1093/jnci/71.6.1281
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
J. McCoy
中科院分区：
文献类型：
--
作者：
J. Varani;E. Lovett;M. Wicha;H. Malinoff;J. McCoy
在培养物中建立了来自2种3-甲基胆蒽诱导的小鼠肿瘤的细胞系，并检查了与Griffonia simplicifolia isolectin B4（GS I-B4）的反应性，Griffonia simplicifolia isolectin B4是一种对末端α-D-半乳吡喃糖残基具有严格特异性的凝集素。事实上，两个群体中的所有细胞都具有强烈的反应性，表明细胞表面存在这种碳水化合物。将两种肿瘤细胞系暴露于含有血型B抗原抗体的人血清。超过99.99%的暴露细胞被这种处理杀死。这并不奇怪，因为末端α-D-吡喃半乳糖残基包含血型B抗原。从少数存活的细胞中，有可能建立抗血型B抗体细胞毒性作用的细胞系。以这种方式独立地获得总共4个细胞系。人血清抗性细胞系显示与GS I-B4没有可检测的反应性，表明已经获得α-D-吡喃半乳糖基缺陷细胞系。比较亲本和变体细胞与抗层粘连蛋白抗体的反应性。两个亲本系在存活状态下通过免疫荧光显示出强反应性，而α-D-吡喃半乳糖基缺陷系显示出无反应性。还比较了亲本和变体系的体外和体内生长。α-D-吡喃半乳糖基缺陷系相对于亲本系具有降低的体外生长能力。更重要的是，与亲本细胞系相比，这些细胞系在注射到同基因小鼠中时的致瘤性显著低于亲本细胞系，并且不会自发转移。
Cell lines from 2 3-methylcholanthrene-induced murine tumors were established in culture and examined for reactivity with Griffonia simplicifolia isolectin B4 (GS I-B4), a lectin that has strict specificity for terminal alpha-D-galactopyranosyl residues. Virtually all of the cells in both populations were strongly reactive, indicating the presence of this carbohydrate on the cell surface. Both tumor lines were exposed to human serum with antibodies to the blood group B antigen. More than 99.99% of the exposed cells were killed by this treatment. This is not surprising, because terminal alpha-D-galactopyranosyl residues comprise the blood group B antigen. From the few surviving cells, it was possible to establish cell lines resistant to the cytotoxic effects of the anti-blood group B antibodies. A total of 4 cell lines were independently obtained in this way. The human serum-resistant lines showed no detectable reactivity with GS I-B4, indicating that alpha-D-galactopyranosyl-deficient cell lines had been obtained. The parent and variant cells were compared for reactivity with anti-laminin antibodies. Both parent lines showed strong reactivity by immunofluorescence in the viable state, whereas the alpha-D-galactopyranosyl-deficient lines showed no reactivity. The parent and variant lines were also compared with regard to in vitro and in vivo growth. The alpha-D-galactopyranosyl-deficient lines had reduced in vitro growth capacity relative to the parent lines. More importantly, in contrast to the parent lines, these lines were significantly less tumorigenic than the parent lines when injected into syngeneic mice and did not metastasize spontaneously.