Salmonella typhimurium recognizes a chemically distinct form of the bacterial quorum-sensing signal Al-2
Salmonella typhimurium recognizes a chemically distinct form of the bacterial quorum-sensing signal Al-2
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DOI：
10.1016/j.molcel.2004.07.020
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发表时间：
2004-09-10
期刊：
影响因子：
16
通讯作者：
Hughson, FM
中科院分区：
文献类型：
--
作者：
Miller, ST;Xavier, KB;Hughson, FM
细菌群体使用细胞间通讯来协调整个群落对生物膜形成、毒力和生物发光等过程的调节。这种现象被称为群体感应，是由称为自诱导物的小分子信号介导的。虽然大多数自诱导物是种特异性自诱导物-2（AI-2），但其首先在海洋细菌哈氏弧菌中被鉴定，由许多革兰氏阴性和革兰氏阳性细菌产生和检测。与其受体蛋白结合的哈维氏弧菌AI-2信号分子的晶体结构揭示了一种不寻常的呋喃糖基硼酸二酯。在这里，我们提出了第二AI-2信号结合蛋白，LSRB从鼠伤寒沙门氏菌的晶体结构。我们发现LsrB结合AI-2信号的化学上不同的形式，（2 R，4S）2-甲基-2，3，3，4-四羟基四氢呋喃（R-THMF），其缺乏硼。我们的研究结果表明，两种不同种类的细菌识别两种不同形式的自诱导物信号，都来自4，5-二羟基-2，3-戊二酮（DPD），并揭示了新的复杂的化学词汇中使用的细菌种间信号。
Bacterial populations use cell-cell communication to coordinate community-wide regulation of processes such as biofilm formation, virulence, and bioluminescence. This phenomenon, termed quorum sensing, is mediated by small molecule signals known as autoinducers. While most autoinducers are species specific autoinducer-2 (AI-2), first identified in the marine bacterium Vibrio harveyi, is produced and detected by many Gram-negative and Gram-positive bacteria. The crystal structure of the V. harveyi AI-2 signaling molecule bound to its receptor protein revealed an unusual furanosyl borate diester. Here, we present the crystal structure of a second AI-2 signal binding protein, LsrB from Salmonella typhimurium. We find that LsrB binds a chemically distinct form of the AI-2 signal, (2R,4S)2-methyl-2,3,3,4-tetrahydroxytetrahydrofuran (R-THMF), that lacks boron. Our results demonstrate that two different species of bacteria recognize two different forms of the autoinducer signal, both derived from 4,5-dihydroxy-2,3-pentanedione (DPD), and reveal new sophistication in the chemical lexicon used by bacteria in interspecies signaling.