Characterization of a human gene conferring sensitivity to infection by gibbon ape leukemia virus.
Characterization of a human gene conferring sensitivity to infection by gibbon ape leukemia virus.
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发表时间：
1990-03
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通讯作者：
O'hara;S. Johann;H. Klinger;D. Blair;H. Rubinson;K. Dunn;P. Sass;S. Vitek;T. Robins
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作者：
O'hara;S. Johann;H. Klinger;D. Blair;H. Rubinson;K. Dunn;P. Sass;S. Vitek;T. Robins
巨猿白血病病毒（GALV）在与特定受体蛋白相互作用后进入细胞。我们已经分离出编码一种蛋白质的人互补DNA（cDNA），当该蛋白质在正常未感染的小鼠NIH 3T3细胞中表达时，赋予这些细胞对GALV感染的特异性敏感性。这是通过将人DNA转染到小鼠细胞中并使用携带耐药基因的逆转录病毒感染来选择推定的受体基因转染子来完成的。然后通过与重复DNA的结合克隆转染的基因组序列，并将其用于分离cDNA克隆。这些cDNA中编码的预测的679个氨基酸序列是一个完整的膜蛋白的特征，因为存在多个潜在的跨膜结构域。对DNA和蛋白质数据库的检索未能揭示与其他已知序列的同源性。因此，分离的序列似乎是新的，并且代表GALV的人受体。正如从病毒的广泛宿主范围所预期的那样，在其他几种测试的脊椎动物物种中发现了该基因的密切相关的同源物。
Gibbon ape leukemia virus (GALV) enters cells following interaction with a specific receptor protein. We have isolated human complementary DNAs (cDNAs) encoding a protein which, when expressed in normally uninfectable mouse NIH3T3 cells, confers on these cells specific sensitivity to infection by GALV. This was done by transfection into mouse cells of human DNA and selection of putative receptor gene transfectants using infection with a retrovirus carrying a drug resistance gene. Transfected genomic sequences were then cloned through their association with repetitive DNA, and these were used to isolate cDNA clones. The predicted 679-amino acid sequence encoded in these cDNAs is characteristic of an integral membrane protein in that multiple potential transmembrane domains are present. Searches of DNA and protein data banks failed to reveal homologies to other known sequences. It thus appears that the sequence isolated is novel and represents the human receptor for GALV. As expected from the wide host range of the virus, closely related homologues of the gene were found in several other vertebrate species tested.