On the Disinfection of Electrochemical Aptamer-Based Sensors.
On the Disinfection of Electrochemical Aptamer-Based Sensors.
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DOI：
10.1149/2754-2726/ac60b2
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发表时间：
2022-03-01
期刊：
影响因子：
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通讯作者：
Plaxco, Kevin W
中科院分区：
文献类型：
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作者：
Chung, Julia;Sepunaru, Lior;Plaxco, Kevin W
基于电化学适配体（EAB）的传感器包括目前报道的唯一生物传感器方法，其同时：（1）独立于其目标的化学或酶反应性，使其通用;（2）连续和实时;以及（3）选择性足以在活体中原位部署。与此相一致的是，体内EAB传感器支持多种药物和代谢物的秒级分辨、实时测量，这表明该方法可能在生物医学研究以及疾病的诊断、治疗和监测中具有价值。然而，要将这些设备应用于长期动物模型，更不用说人类患者，需要它们没有任何显著的病原体负荷。因此，我们在这里描述了EAB传感器与标准灭菌和高水平消毒技术的兼容性。这样做，我们发现，虽然许多导致显着的传感器降解，用CIDEX OPA（0.55%邻苯二甲醛）处理导致有效的消毒，而不会导致任何可检测到的传感器性能损失。
Electrochemical aptamer-based (EAB) sensors encompass the only biosensor approach yet reported that is simultaneously: (1) independent of the chemical or enzymatic reactivity of its target, rendering it general; (2) continuous and real-time; and (3) selective enough to deploy in situ in the living body. Consistent with this, in vivo EAB sensors supporting the seconds-resolved, real-time measurement of multiple drugs and metabolites have been reported, suggesting the approach may prove of value in biomedical research and the diagnosis, treatment, and monitoring of disease. However, to apply these devices in long-duration animal models, much less in human patients, requires that they be free of any significant pathogen load. Thus motivated, here we have characterized the compatibility of EAB sensors with standard sterilization and high-level disinfection techniques. Doing so, we find that, while many lead to significant sensor degradation, treatment with CIDEX OPA (0.55% ortho-phthalaldehyde) leads to effective disinfection without causing any detectable loss in sensor performance.