ISCHEMIA INDUCES THE EXPRESSION OF THE PLATELET-DERIVED GROWTH FACTOR-B CHAIN IN NEURONS AND BRAIN MACROPHAGES IN-VIVO
ISCHEMIA INDUCES THE EXPRESSION OF THE PLATELET-DERIVED GROWTH FACTOR-B CHAIN IN NEURONS AND BRAIN MACROPHAGES IN-VIVO
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DOI：
10.1038/jcbfm.1994.102
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发表时间：
1994-09-01
影响因子：
6.3
通讯作者：
HAZAMA, F
中科院分区：
文献类型：
--
作者：
IIHARA, K;SASAHARA, M;HAZAMA, F
为了阐明血小板衍生生长因子 (PDGF)-B 链在大脑中的作用，我们检测了其在局灶性缺血的大鼠大脑中的表达。自发性高血压大鼠（SHR）大脑中动脉和颈总动脉的永久性串联闭塞诱导局灶性缺血。 Northern 分析表明，缺血会短暂增加受损新皮质中 PDGF-B 链的 mRNA 表达，但不会增加 PDGF-A 链的 mRNA 表达。 B链较大的转录本(3.5kb)在16小时内逐渐增加至三倍，而B链较小的转录本(2.6kb)在4小时内显着增加六倍。免疫组织化学显示，从 16 小时到第 4-7 天，梗塞和梗塞周围区域神经元的免疫反应性增强，在 24 小时达到峰值。此外，梗死区中积聚的脑巨噬细胞在第 2 天至第 14 天期间在其核周区域显示出强烈的免疫染色，在第 5 天至第 6 天达到峰值。本研究表明，缺血首先在神经元中，然后在脑巨噬细胞中诱导 PDGF-B 链的表达，并表明 PDGF-B 链在受伤大脑的愈合过程中发挥着重要作用。
To elucidate the role of the platelet-derived growth factor (PDGF)-B chain in the brain, we examined its expression in rat brains with focal ischemia. Focal ischemia was induced by permanent tandem occlusion of the middle cerebral and common carotid arteries in spontaneously hypertensive rats (SHRs). Northern analysis demonstrated that ischemia transiently increased mRNA expression of the PDGF-B chain, but not the PDGF-A chain, in the injured neocortex. The larger transcript (3.5 kb) of the B chain gradually increased to threefold by 16 h, whereas the smaller transcript (2.6 kb) of the B chain markedly increased sixfold by 4 h. Immunohistochemistry revealed enhanced immunoreactivity in the neurons in the infarct and in the periinfarct area from 16 h to days 4-7, with a peak at 24 h. Furthermore, the brain macrophages that accumulated in the infarct showed intense immunostaining in their perinuclear region from days 2 to 14, with a peak at days 5-6. The present study demonstrates that ischemia induces the expression of the PDGF-B chain, first in neurons and later in brain macrophages, and suggests an important role of the PDGF-B chain in the healing process of the injured brain.