Analgesic effect of Coptis chinensis rhizomes (Coptidis Rhizoma) extract on rat model of irritable bowel syndrome.
Analgesic effect of Coptis chinensis rhizomes (Coptidis Rhizoma) extract on rat model of irritable bowel syndrome.
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DOI：
10.1016/j.jep.2011.04.007
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发表时间：
2011-06-01
影响因子：
5.4
通讯作者：
Che, Chuntao
中科院分区：
文献类型：
--
作者：
Tjong, Yungwui;Ip, Siupo;Lao, Lixing;Fong, Harry H. S.;Sung, Joseph J. Y.;Berman, Brian;Che, Chuntao
关键词：
黄连(Coptis chinese Rhizoma，CR)，又名黄连，是传统中草药中用于缓解腹痛和腹泻的常见成分。然而，CR提取物治疗肠易激综合征的作用机制尚不清楚。因此，本研究旨在探讨黄连提取物对新生儿母体分离(NMS)所致大鼠内脏痛敏的影响及其可能的作用机制。雄性SD大鼠从出生后第2天至第21天每天进行3小时的母体分离，形成NMS组。对照组为未分离的正常(N)大鼠。从第60天开始，试验组和对照组分别给予CR(0.3、0.8和1.3g/kg)或赋形剂(0.5%羧甲基纤维素溶液)灌胃7d。对NMS大鼠结肠扩张后的肌电(EMG)信号进行了测量，发现NMS大鼠较未分离(N)大鼠具有更低的痛阈值和更高的EMG活性。CR剂量依赖性地增加了NMS大鼠的痛阈值反应，并减弱了肌电活动。酶免疫测定研究表明，与Veh(对照组)相比，CR处理显著降低了NMS大鼠远端结肠的5-羟色胺(5-HT)浓度。实时定量PCR和Western-blotting研究表明，与Veh组相比，CR治疗显著降低了NMS诱导的CCK的表达。这些结果提示，黄连提取物能显著减轻内脏痛，其机制可能与减少大鼠远端结肠5-羟色胺的释放和CCK的表达有关。
Coptis chinensis rhizomes (Coptidis Rhizoma, CR), also known as “Huang Lian”, is a common component of traditional Chinese herbal formulae used for the relief of abdominal pain and diarrhea. Yet, the action mechanism of CR extract in the treatment of irritable bowel syndrome is unknown. Thus, the aim of our present study is to investigate the effect of CR extract on neonatal maternal separation (NMS)-induced visceral hyperalgesia in rats and its underlying action mechanisms. Male Sprague-Dawley rats were subjected to 3-hr daily maternal separation from postnatal day 2 to day 21 to form the NMS group. The control group consists of unseparated normal (N) rats. From day 60, rats were administrated CR (0.3, 0.8 and 1.3 g/Kg) or Vehicle (Veh; 0.5% carboxymethylcellulose solution) orally for 7 days for the test and control groups, respectively. Electromyogram (EMG) signals in response to colonic distension were measured with the NMS rats showing lower pain threshold and increased EMG activity than those of the unseparated (N) rats. CR dose-dependently increased pain threshold response and attenuated EMG activity in the NMS rats. An enzymatic immunoassay study showed that CR treatment significantly reduced the serotonin (5HT) concentration from the distal colon of NMS rats compared to the Veh (control) group. Real-time quantitative PCR and Western-blotting studies showed that CR treatment substantially reduced NMS induced cholecystokinin (CCK) expression compared with the Veh group. These results suggest that CR extract robustly reduces visceral pain that may be mediated via the mechanism of decreasing 5HT release and CCK expression in the distal colon of rats.
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影响因子：
5.4
作者：
Kim, Jong Min;Jung, Hyun Ah;Lee, Na Gyong
通讯作者：
Lee, Na Gyong
影响因子：
3.5
作者：
Gershon, M. D.;Liu, M.-T.
通讯作者：
Liu, M.-T.
影响因子：
2
作者：
Jung, Hyun Ah;Min, Byung-Sun;Choi, Jae Sue
通讯作者：
Choi, Jae Sue
影响因子：
2.7
作者：
Greisen, MH;Bolwig, TG;Wörtwein, G
通讯作者：
Wörtwein, G
DOI：
10.1124/jpet.107.132670
发表时间：
2008-04-01
影响因子：
3.5
作者：
Liu, Qingyun;Yang, Qi;Shi, Zhi-Cai
通讯作者：
Shi, Zhi-Cai