IDENTIFICATION OF 2 MESSENGER PPAR RELATED RECEPTORS AND EVIDENCE FOR THE EXISTENCE OF 5 SUBFAMILY MEMBERS
IDENTIFICATION OF 2 MESSENGER PPAR RELATED RECEPTORS AND EVIDENCE FOR THE EXISTENCE OF 5 SUBFAMILY MEMBERS
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DOI：
10.1006/bbrc.1993.2302
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发表时间：
1993-10-29
影响因子：
3.1
通讯作者：
OMALLEY, BW
中科院分区：
文献类型：
--
作者：
CHEN, F;LAW, SW;OMALLEY, BW
另外两种形式的小鼠过氧化物酶体增殖物激活受体已被确定从cDNA文库来源于小鼠组织使用低严格性交叉杂交筛选方法。一种是mNUCI，其在其DNA结合结构域中与hNUCI具有97%的氨基酸序列同一性。另一个是mPPARγ。mPPARγ的全长cDNA编码一个长为470个氨基酸的蛋白质。与xPPARγ的氨基酸序列同源性为75%，其中DNA结合区和配体结合区的同源性最高，分别为97%和86%。两种新形式mPPAR的发现和最近hPPAR的克隆为过氧化物酶体增殖物激活受体亚家族中至少存在五种不同形式的受体提供了证据。
Two additional forms of mouse peroxisome proliferator activated receptor have been identified from cDNA libraries derived from mouse tissues using a low stringency cross-hybridization screening method. One is mNUCI, which has 97% amino acid sequence identity with hNUCI in its DNA binding domain. The other one is mPPARγ. The full-length cDNA of mPPARγ encodes a protein of 470 amino acids in length. The overall amino acid sequence identity is 75% as compared with xPPARγ, with the highest homology in the DNA binding region and ligand binding region, 97% and 86% identity, respectively. The discovery of two new forms of mPPAR and the recent cloning of hPPAR provide evidence for the existence of at least five different forms of receptor in the peroxisome proliferator activated receptor subfamily.