Plasma Treatment Effects on Oral Candida albicans Biofilms.
Plasma Treatment Effects on Oral Candida albicans Biofilms.
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DOI：
10.31487/j.dobcr.2021.02.05
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Yu, Qingsong
中科院分区：
文献类型：
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作者：
Hong, Qing;Dong, Xiaoqing;Chen, Meng;Sun, Hongmin;Hong, Liang;Wang, Yong;Yu, Qingsong
本研究的目的是评估等离子体处理对口腔真菌生物膜的影响。在48孔板上形成白色念珠菌生物膜以用作口腔真菌生物膜的模型。与血浆处理相比，使用0.2%二葡萄糖酸氯己定（CHX）处理作为阳性对照。采用MTT比色法和激光共聚焦显微镜（CLSM）观察各组的治疗效果。随着等离子体功率从6mA增加到8mA，等离子体暴露时间从2min延长到10min，白色念珠菌的存活率从52%下降到27%。扫描电子显微镜（SEM）检查表明，严重的细胞损伤等离子体处理。总之，低温等离子体处理在灭活白色念珠菌生物膜方面是有效的，因此提供了一种有前途的替代方案来消毒口腔真菌生物膜。
The objective of this study is to evaluate the plasma treatment effects on oral fungal biofilms. Candida albicans biofilms were developed on the 48-well plate to serve as a model of oral fungal biofilm. The treatment of 0.2% chlorhexidine digluconate (CHX) was used as a positive control compared with plasma treatments. The efficacy of treatments was determined by 3-(4,5-dimethylazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and confocal laser scanning microscope (CLSM). The survival percentage of Candida albicans decreased from 52% to 27% as the plasma power increased from 6mA to 8mA and plasma exposure time extended from 2 min to 10 min. Moreover, it was found that there is a synergistic effect of the combination of plasma and CHX treatments. Scanning electron microscopy (SEM) examination indicated severe cell damages resulting from plasma treatment. In conclusion, the low-temperature plasma treatment is effective in deactivating Candida albicans biofilms and thus provides a promising alternative to disinfect oral fungal biofilms.