Metabolic Profile in Neonatal Pig Hearts.
Metabolic Profile in Neonatal Pig Hearts.
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新生儿猪心中的代谢谱。
DOI：
10.3389/fcvm.2021.763984
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发表时间：
2021
影响因子：
3.6
通讯作者：
Zhu W
中科院分区：
文献类型：
--
作者：
Li P;Li F;Tang L;Zhang W;Jin Y;Gu H;Zhu W
我们使用靶向液相色谱串联质谱法评估了出生后第1、3、7和28天（分别为P1、P3、P7和P28）猪心脏的代谢谱。我们的数据显示，P1和P28心脏中检测到的代谢物有明显的分离。当心肌细胞保持高细胞周期活性时，在P1心脏中观察到核苷酸和蛋白质的活跃合成代谢。然而，当心肌细胞退出细胞周期时，P28心脏中观察到活跃的翻译后蛋白修饰，从葡萄糖到脂肪酸的代谢转换，以及胶原蛋白与总蛋白的比例降低。
We evaluated the metabolic profile in pig hearts at postnatal day 1, 3, 7, and 28 (P1, P3, P7, and P28, respectively) using a targeted liquid chromatography tandem mass spectrometry assay. Our data showed that there is a clear separation of the detected metabolites in P1 vs. P28 hearts. Active anabolisms of nucleotide and proteins were observed in P1 hearts when cardiomyocytes retain high cell cycle activity. However, the active posttranslational protein modification, metabolic switch from glucose to fatty acids, and the reduced ratio of collagen to total protein were observed in P28 hearts when cardiomyocytes withdraw from cell cycle.