Flow cytometry analysis of the circulating haemocytes from Biomphalaria glabrata and Biomphalaria tenagophila following Schistosoma mansoni infection
Flow cytometry analysis of the circulating haemocytes from Biomphalaria glabrata and Biomphalaria tenagophila following Schistosoma mansoni infection
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DOI：
10.1017/s0031182008005155
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发表时间：
2009-01-01
期刊：
影响因子：
2.4
通讯作者：
Negrao-Correa, D.
中科院分区：
文献类型：
--
作者：
Martins-Souza, R. L.;Pereira, C. A. J.;Negrao-Correa, D.
为了进一步表征血细胞亚群在双脐螺，我们已经进行了详细的流式细胞仪分析的全血淋巴细胞成分使用多参数双色，标记程序。溴化铵/吖啶橙子荧光特征用于首先选择活血细胞，然后基于激光散射特性（前向散射-FSC和侧向散射-SSC）进行流式细胞术形态测定分析。结果表明，B. glabrata（BG-BII，对S. mansoni）和B. tenagophila（BT-CF，中度敏感，BT-Taim，对S. mansoni）具有3个主要的循环血细胞亚群，称为小、中和大血细胞。BG-BII中小血细胞的频率较高，而中等血细胞是两种B中最丰富的细胞类型。tenagophila菌株。曼氏血吸虫感染导致早期大、中型血细胞减少，随后小血细胞增加。虽然寄生虫感染引起血细胞的变化，在所有的双脐螺属菌株，反应是特别强烈的BT-泰姆，抗寄生虫的蜗牛。有趣的是，吸虫感染引起的变化，血细胞的颗粒少，而不是在那些，更多的颗粒配置文件。结果表明，在B. Taim株tenagophila的循环血细胞，尤其是颗粒较少的中、高亚群，对S.这表明该细胞亚群将参与在该蜗牛株中观察到的早期寄生虫破坏。
Aiming to further characterize the haemocyte subsets in Biomphalaria snails, we have performed a detailed flow cytometric analysis of whole haemolymph cellular components using a multiparametric dual colour, labelling procedure. Ethidium bromide/acridine orange fluorescence features were used to first select viable haemocytes followed by flow cytometric morphometric analysis based on the laser scatter properties (forward scatter-FSC and side scatter-SSC). Our findings demonstrated that B. glabrata (BG-BII, highly Susceptible to S. mansoni) and 2 strains of B. tenagophila (BT-CF, moderately Susceptible and BT-Taim, resistant to S. mansoni) have 3 major circulating haemocyte Subsets, referred to as small, medium and large haemocytes. The frequency of small haemocytes was higher in BG-BII, while medium haemocytes were the most abundant cell-type in both B. tenagophila strains. Schistosoma mansoni infection resulted in early reduction of large and medium circulating haernocytes followed by an increase of small haernocytes. Although parasite infection induced haemocyte alterations in all Biomphalaria strains, the response was particularly intense in BT-Taim, the parasite-resistant snail. Interestingly, the trematode infection induces changes in haemocytes with less granular rather than in those,with more granular profile. The results indicated that, in B. tenagophila of Taim strain, circulating haemocytes, especially the medium and high Subset with less granular profile, are very reactive cells upon S. mansoni infection, Suggesting that this cell subset would participate in the early parasite destruction observed in this snail strain.