Studies on a radioimmunoassay for human erythopoietin.
Studies on a radioimmunoassay for human erythopoietin.
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人促红细胞生成素放射免疫分析的研究。
DOI：
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发表时间：
1975
期刊：
影响因子：
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通讯作者：
J. Fisher
中科院分区：
文献类型：
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作者：
J. Lertora;P. Dargon;A. Rege;J. Fisher
使用氯胺-T 方法用 Na'125I 标记高度纯化的促红细胞生成素 (ESF) 制剂（每毫克蛋白质 12,000 单位）。通过 Sephadex G-150 分级分离将未损坏的免疫反应标记的 ESF 与损坏的、非免疫接收的 ESF 分离。这种未受损的免疫反应性ESF被用于人类促红细胞生成素的放射免疫测定。使用双抗体技术实现结合抗原与游离抗原的分离。在 1:1500 的抗血清稀释度下观察到大约 55% 的结合。该测定似乎足够灵敏，可以检测低至 0.025 毫单位的国际参考制剂促红细胞生成素。正常和贫血尿毒症人类受试者中 thid 激素的估计水平表明，尿毒症贫血患者的免疫反应性血清促红细胞生成素水平高于正常水平。
A highly purified erythropoietin (ESF) preparation (12,000 units per milligram of protein) was labeled with Na'125I using the Chloramine-T method. Undamaged immunoreative labeled ESF was separated from the damaged, nonimmunologically receiveESF by Sephadex G-150 fractionation. This undamaged immunreactive ESF was usedin radioimmunoassay for human erythropoietin. Separation of bound from free antigen was acheived using the double-antibody technique. Approximately 55 per cent binding wasobserved at an antiserum dilution of 1:1500. This assay appears to be sensitive enough to detect as little as 0.025 milliunits of the International Reference Preparation erythropoietin. The estimated levels of thid hormone in normal and anemic uremic human subjects suggests that immunoreactive serum erythropoietin levels are elevated above normal in anemia of uremia.