The gold-nanoparticle-based surface plasmon resonance light scattering and visual DNA aptasensor for lysozyme
The gold-nanoparticle-based surface plasmon resonance light scattering and visual DNA aptasensor for lysozyme
复制标题
基于金纳米颗粒的溶菌酶表面等离子体共振光散射和视觉DNA适体传感器
DOI：
10.1007/s00216-011-4943-1
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发表时间：
2011-06-01
影响因子：
4.3
通讯作者：
Li, Na
中科院分区：
文献类型：
--
作者：
Wang, Xinyi;Xu, Yao;Li, Na
我们开发了一种新的简单和灵敏的检测溶菌酶的金纳米粒子等离子体共振光散射（PRLS）测量和裸眼检测的基础上，首次使用溶菌酶DNA适体作为识别元件。溶菌酶DNA适体可以在高离子强度下稳定金纳米粒子。将溶菌酶引入到系统中容易触发AuNPs的聚集，产生溶液的红到蓝颜色变化，红移等离子体吸收，以及增强的等离子体共振光散射。发现0.7 nM AuNP的线性范围为0.2 nM ~ 4 nM，1.4 nM AuNP的线性范围为0.3 nM ~ 6 nM，2.1 nM AuNP的线性范围为0.6 nM ~ 8 nM。约0.1 nM溶菌酶可产生可观察到的PRLS信号增强。对于视觉检测，1 nM溶菌酶可以产生非常独特的颜色变化。模拟唾液和稀释尿样的回收率均令人满意，表明该方法具有潜在的临床样品的分析。简单和高灵敏度与许多实验室的资源和需求相一致，使该方法成为常规分析的良好选择。图DNA适体稳定的AuNPs聚集的示意图描述和演示用于溶菌酶的比色和PRLS传感。
We developed a new simple and sensitive assay for lysozyme based on gold nanoparticle plasmon resonance light scattering (PRLS) measurement and naked-eye detection using for the first time the lysozyme DNA aptamer as the recognition element. Lysozyme DNA aptamer could stabilize gold nanoparticles (AuNPs) at high ionic strength. Introducing lysozyme to the system easily triggered the aggregation of AuNPs, producing a red-to-blue color change of the solution, red-shifted plasmon absorption, and enhanced plasmon resonance light scattering. The linear range was found to be 0.2∼4 nM for 0.7 nM AuNPs, 0.3∼6 nM for 1.4 nM AuNPs and 0.6∼8 nM for 2.1 nM AuNPs. About 0.1 nM lysozyme can produce an observable enhancement of PRLS signal. For visual detection, 1 nM lysozyme can produce a very distinctive color change. Satisfactory recoveries were obtained for simulated saliva and diluted urine samples, indicating that the method has potential for analyses of clinical samples. The simplicity and high sensitivity that are consistent with the resources and needs of many laboratories makes this method a good choice for routine analysis.FigureSchematic description and demonstration of aggregation of DNA aptamer stabalized AuNPs for colorimetric and PRLS sensing of lysozyme.