Biomarker and competing endogenous RNA potential of tumor-specific long noncoding RNA in chromophobe renal cell carcinoma.
Biomarker and competing endogenous RNA potential of tumor-specific long noncoding RNA in chromophobe renal cell carcinoma.
复制标题
嫌色肾细胞癌中肿瘤特异性长非编码RNA的生物标志物和竞争性内源RNA潜力
DOI：
10.2147/ott.s116392
复制
发表时间：
2016
影响因子：
4
通讯作者：
Yang Q
中科院分区：
文献类型：
--
作者：
He HT;Xu M;Kuang Y;Han XY;Wang MQ;Yang Q
背景越来越多的证据表明，长非编码RNA(LncRNAs)在肿瘤的发生发展中起着重要作用。然而，它们在嫌色肾细胞癌(ChRCC)中的作用还不完全清楚。方法分析LncRNA、microRNA和蛋白质编码RNA的表达谱，并结合癌症基因组图谱数据库收集的59例原发性慢性肾癌患者的临床资料，寻找预测预后的LncRNA生物标志物。我们还利用生物信息学的方法构建了lncRNA-microRNA-mRNAs共表达网络(竞争性内源RNAs网络)。结果发现142个≥在癌组织和正常组织中差异表达(倍数变化LncRNA 1.5，P<；0.001)。其中12个LncRNA的表达与临床特征有差异(倍变≥1.5，P<；0.0 1)。此外，7个LncRNAs(COL18A1-AS、BRE-AS1、SNHG7、TMEM51-AS1、C21orf62-AS1、LINC00336和LINC00882)与总生存率显著相关(对数等级P<；0.05)。构建了一个含有16个lncRNAs、18个miRNAs和168个蛋白质编码RNAs的竞争性内源RNA网络。结论我们的结果发现了与chRCC进展和预后相关的特异性lncRNAs，并呈现出肿瘤中lncRNAs竞争的内源性RNA潜能。
Background Accumulating evidence suggests long noncoding RNAs (lncRNAs) play important roles in the initiation and progression of cancers. However, their functions in chromophobe renal cell carcinoma (chRCC) are not fully understood. Methods We analyzed the expression profiles of lncRNA, microRNA, and protein-coding RNA, along with the clinical information of 59 primary chRCC patients collected from The Cancer Genome Atlas database to identify lncRNA biomarkers for prognosis. We also constructed an lncRNA–microRNA–mRNA coexpression network (competitive endogenous RNAs network) by bioinformational approach. Results One hundred and forty-two lncRNAs were found to be differentially expressed between the cancer and normal tissues (fold change ≥1.5, P<0.001). Among them, 12 lncRNAs were also differentially expressed with the corresponding clinical characteristics (fold change ≥1.5, P<0.01). Besides, 7 lncRNAs (COL18A1-AS, BRE-AS1, SNHG7, TMEM51-AS1, C21orf62-AS1, LINC00336, and LINC00882) were identified to be significantly correlated with overall survival (log-rank P<0.05). A competitive endogenous RNA network in chRCC containing 16 lncRNAs, 18 miRNAs, and 168 protein-coding RNAs was constructed. Conclusion Our results identified specific lncRNAs associated with chRCC progression and prognosis, and presented competing endogenous RNA potential of lncRNAs in the tumor.