Transcriptional shifts in delignification‐defective mutants of the white‐rot fungus Pleurotus?ostreatus
Transcriptional shifts in delignification‐defective mutants of the white‐rot fungus Pleurotus?ostreatus
复制标题
白腐真菌平菇脱木质素缺陷突变体的转录变化
DOI：
10.1002/1873-3468.13890
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发表时间：
2020
期刊：
影响因子：
3.5
通讯作者：
Honda Yoichi
中科院分区：
文献类型：
--
作者：
Wu Hongli;Nakazawa Takehito;Takenaka Atsuki;Kodera Rina;Morimoto Ryota;Sakamoto Masahiro;Honda Yoichi
白腐菌能有效降解木质素，因此在全球碳循环中起着举足轻重的作用。然而，木质素降解的机理在很大程度上还不清楚。最近，四个基因的突变，即wtr1、chd1、pex1和gat1，被证明取消了平菇降解木材木质素的能力。在这项研究中，我们进行了比较转录组分析，以确定在木质素分解缺陷突变株中差异表达的基因。在亲本菌株中高表达的可能的木质素分解基因在突变菌株中显著下调。相反，在Δpex1和Δgat1菌株中，许多假定的纤维素分解和木聚糖酶降解基因上调。鉴定突变菌株中的转录变化可以为木质纤维分解基因INP.ostreatus的调控机制提供新的见解。
White‐rot fungi efficiently degrade lignin and, thus, play a pivotal role in the global carbon cycle. However, the mechanisms of lignin degradation are largely unknown. Recently, mutations in four genes, namelywtr1,chd1,pex1, andgat1, were shown to abrogate the wood lignin‐degrading ability ofPleurotus ostreatus. In this study, we conducted a comparative transcriptome analysis to identify genes that are differentially expressed in ligninolysis‐deficient mutant strains. Putative ligninolytic genes that are highly expressed in parental strains are significantly downregulated in the mutant strains. On the contrary, many putative cellulolytic and xylanolytic genes are upregulated in thechd1‐1, Δpex1, and Δgat1strains. Identifying transcriptional alterations in mutant strains could provide new insights into the regulatory mechanisms of lignocellulolytic genes inP. ostreatus.