Expression of the thyroid hormone receptor gene, erbA alpha, in B lymphocytes: alternative mRNA processing is independent of differentiation but correlates with antisense RNA levels
Expression of the thyroid hormone receptor gene, erbA alpha, in B lymphocytes: alternative mRNA processing is independent of differentiation but correlates with antisense RNA levels
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DOI：
10.1093/nar/25.21.4296
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发表时间：
1997-11-01
影响因子：
14.9
通讯作者：
Munroe, SH
中科院分区：
文献类型：
--
作者：
Hastings, ML;Milcarek, C;Munroe, SH
erbA α基因编码两种α-甲状腺激素受体亚型，TR α 1和TR α 2，它们来自交替加工的mRNA，erbA α 1（α 1）和erb α 2（α 2）。这些mRNA的剪接和可变多聚腺苷酸化模式类似于编码不同形式的免疫球蛋白重链的mRNA的剪接和可变多聚腺苷酸化模式，其在B细胞分化期间在可变加工水平上受到调节，本研究检测了代表4个分化阶段的8个B细胞系中erbA α mRNA的水平，以确定交替加工的α 1和α 2 mRNA的调节是否平行结果表明，α 1和α 2 mRNA的表达模式与免疫球蛋白重链mRNA的表达模式明显不同，B细胞系在不同的分化阶段显示出特征性的α 1/α 2 mRNA比率。此外，发现重叠基因Rev-ErbA α（RevErb）的表达与α 1/α 2 mRNA比率的增加强烈相关。这些结果表明，erbA α mRNA的替代加工受与调节免疫球蛋白mRNA不同的机制调节，RevErb和erbA α mRNA之间的相关性与α 2通过与互补RevErb mRNA的反义相互作用的负调控一致。
The erbA alpha gene encodes two alpha-thyroid hormone receptor isoforms, TR alpha 1 and TR alpha 2, which arise from alternatively processed mRNAs, erbA alpha 1 (alpha 1) and erb alpha 2 (alpha 2). The splicing and alternative polyadenylation patterns of these mRNAs resemble that of mRNAs encoding different forms of immunoglobulin heavy chains, which are regulated at the level of alternative processing during B cell differentiation, This study examines the levels of erbA alpha mRNA in eight B cell lines representing four stages of differentiation in order to determine whether regulation of the alternatively processed alpha 1 and alpha 2 mRNAs parallels the processing of immunoglobulin heavy chain mRNAs, Results show that the pattern of alpha 1 and alpha 2 mRNA expression is clearly different from that observed for immunoglobulin heavy chain mRNAs, B cell lines display characteristic ratios of alpha 1/alpha 2 mRNA at distinct stages of differentiation. Furthermore, expression of an overlapping gene, Rev-ErbA alpha (RevErb), was found to correlate strongly with an increase in the ratio of alpha 1/alpha 2 mRNA, These results suggest that alternative processing of erbA alpha mRNAs is regulated by a mechanism which is distinct from that regulating immunoglobulin mRNA, The correlation between RevErb and erbA alpha mRNA is consistent with negative regulation of alpha 2 via antisense interactions with the complementary RevErb mRNA.