Low-Dose Radiation Exposure with 56MnO2 Powder Changes Gene Expressions in the Testes and the Prostate in Rats
Low-Dose Radiation Exposure with 56MnO2 Powder Changes Gene Expressions in the Testes and the Prostate in Rats
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DOI：
10.3390/ijms21144989
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发表时间：
2020-07-01
影响因子：
5.6
通讯作者：
Hoshi, Masaharu
中科院分区：
文献类型：
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作者：
Fujimoto, Nariaki;Amantayeva, Gaukhar;Hoshi, Masaharu
为了研究原子弹爆炸后土壤中主要放射性同位素粉尘放射性(56)MnO(2)粉末内照射对男性生殖功能的生物学影响，检测了睾丸和前列腺的基因表达。十周大的雄性 Wistar 大鼠暴露于三种剂量的放射性（56）MnO（2）粉末（全身剂量为 41-100 mGy）、稳定的 MnO（2）粉末或外部（60）Co 伽马射线（2 Gy）。在暴露后第3天和第61天对动物进行尸检。通过Q-RT-PCR对睾丸标记蛋白基因和前列腺分泌蛋白基因的mRNA表达进行定量。暴露后第3天，(56)MnO(2)暴露组中类固醇生成相关酶Cyp17a1和Hsd3b1的睾丸基因表达下降。生殖细胞特异性的 Spag4 和 Zpbp mRNA 水平也有所降低。在暴露后第61天，暴露于最高剂量(56)MnO(2)组的睾丸中Cyp11a1基因表达显着降低，而另一种类固醇生成相关的StAR基因mRNA水平在(60)Co伽马射线组中降低。第 61 天，各组间 Spag4 和 Zpbp mRNA 水平没有差异。暴露后任何组的睾丸均未观察到组织病理学变化。暴露后第61天，前列腺蛋白基因（包括CRP1、KS3和PSP94）的表达在(56)MnO(2)暴露组以及(60)Co伽马射线组中显着降低。这些数据表明，内部暴露于(56)MnO(2)粉末，剂量低于100 mGy，影响睾丸和前列腺中的基因表达，而2 Gy的外部伽玛射线照射效果较差。
To investigate the biological effects of internal exposure of radioactive(56)MnO(2)powder, the major radioisotope dust in the soil after atomic bomb explosions, on male reproductive function, the gene expression of the testes and the prostate was examined. Ten-week-old male Wistar rats were exposed to three doses of radioactive(56)MnO(2)powder (41-100 mGy in whole body doses), stable MnO(2)powder, or external(60)Co gamma-rays (2 Gy). Animals were necropsied on Days 3 and 61 postexposure. The mRNA expressions of testicular marker protein genes and prostatic secretory protein genes were quantified by Q-RT-PCR. On Day 3 postexposure, the testicular gene expressions of steroidogenesis-related enzymes, Cyp17a1 and Hsd3b1, decreased in(56)MnO(2)-exposed groups. Germ cell-specific Spag4 and Zpbp mRNA levels were also reduced. On postexposure Day 61, the Cyp11a1 gene expression became significantly reduced in the testes in the group exposed to the highest dose of(56)MnO(2), while another steroidogenesis-related StAR gene mRNA level reduced in the(60)Co gamma-rays group. There were no differences in Spag4 and Zpbp mRNA levels among groups on Day 61. No histopathological changes were observed in the testes in any group following exposure. Expression in the prostatic protein genes, including CRP1, KS3, and PSP94, significantly decreased in(56)MnO(2)-exposed groups as well as in the(60)Co gamma-rays group on Day 61 postexposure. These data suggest that the internal exposure to(56)MnO(2)powder, at doses of less than 100 mGy, affected the gene expressions in the testis and the prostate, while 2 Gy of external gamma-irradiation was less effective.