BSA-capped gold nanoclusters as potential theragnostic for skin diseases: Photoactivation, skin penetration, in vitro, and in vivo toxicity
BSA-capped gold nanoclusters as potential theragnostic for skin diseases: Photoactivation, skin penetration, in vitro, and in vivo toxicity
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DOI：
10.1016/j.msec.2020.110891
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发表时间：
2020-07-01
影响因子：
7.9
通讯作者：
Soler-Illia, Galo J. A. A.
中科院分区：
文献类型：
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作者：
Lillo, Cristian R.;Calienni, M. Natalia;Soler-Illia, Galo J. A. A.
BSA包覆的金纳米团簇是一种很有前途的治疗学系统，可以激发产生荧光发射和活性氧。虽然它们的合成和光致发光特性已经得到很好的描述，但为了推进健康应用，需要关于它们作为光敏剂的用途的更准确的信息。在这项工作中，我们获得了BSA覆盖的金纳米团簇，并通过不同的技术表征了它们的电子物理。在照射后检测到单线态氧的产生，这足以对两种细胞系产生毒性。值得注意的是，内部的能量转移，可能是由于存在较小的纳米簇和BSA在系统中的氧化残基的贡献，在UV范围内激发后，在640 nm附近引起荧光发射。此外，该系统能够穿透角质层以外的人类皮肤，这增强了这些纳米团簇作为双功能光动力治疗效应物和生物标志物的潜力，可应用于多种皮肤疾病。在不存在辐射的情况下，BSA覆盖的金纳米团簇在体外没有引起毒性，而它们对斑马鱼的体内模型的毒性作用被确定。
BSA-capped gold nanoclusters are promising theragnostic systems that can be excited to render both fluorescence emission and reactive oxygen species. Although their synthesis and photoluminescence properties are already well described, more accurate information about their use as photosensitizers is required in order to advance towards health applications. In this work, we have obtained BSA-capped gold nanoclusters and characterized their photophysics by different techniques. Singlet oxygen production was detected upon irradiation, which was enough to produce toxicity on two cell lines. Remarkably, an internal energy transfer, probably due to the presence of smaller nanoclusters and the contribution of oxidized residues of BSA in the system, caused fluorescence emission near 640 nm after excitation in the UV range. Additionally, the system was capable of penetrating human skin beyond the stratum corneum, which enhances the potential of these nanoclusters as bifunctional photodynamic therapy effectors and biomarkers with application in a diversity of skin diseases. In the absence of radiation, BSA-capped gold nanoclusters did not cause toxicity in vitro, while their toxic effect on an in vivo model as zebrafish was determined.