Responses of a population of antennal olfactory receptor cells in the female moth Manduca sexta to plant-associated volatile organic compounds
Responses of a population of antennal olfactory receptor cells in the female moth Manduca sexta to plant-associated volatile organic compounds
复制标题
DOI：
10.1007/s003590000165
复制
发表时间：
2001-02
期刊：
影响因子：
--
通讯作者：
V. Shields;J. Hildebrand
中科院分区：
文献类型：
--
作者：
V. Shields;J. Hildebrand
用A型毛状感受器在雌性狮身人面像触角上进行细胞外电生理记录。天线的一个环圈承载着大约1100个这样的感受器，每个感受器由两个嗅觉感受器细胞支配。我们测试了这些受体细胞对一组102种挥发性化合物以及三种植物气味混合物的反应，并可以区分三种不同功能类型的A型毛状感受器。受体细胞的一个子集表现出明显狭窄的分子接受范围，只对一两种萜类气味有强烈的反应。第二个亚组完全由芳烃激活，对两到七种气味有强烈的反应。第三个亚组具有广泛的分子接受范围，对属于几个化学类别的气味有强烈的反应。我们还发现，受体细胞对任何测试的气味都没有反应，但自发地活跃。某些气味在一些感受器上引起兴奋反应，而在另一些感受器上引起抑制反应，一些感受器细胞被某些气味强烈地兴奋，但被另一些感受器抑制。用罗丹明-葡聚糖对A型毛状感受器的受体细胞群进行浸染，结果表明，它们的轴突主要投射到触角小叶的大的雌性小球。
Extracellular electrophysiological recordings were made from individual type-A trichoid sensilla on the antenna of the female sphinx mothManduca sexta. A single annulus of the antenna bears about 1,100 of these sensilla, and each is innervated by two olfactory receptor cells. We tested the responses of these receptor cells to a panel of 102 volatile compounds, as well as three plant-derived odor mixtures, and could discern three different functional types of type-A trichoid sensilla. One subset of receptor cells exhibited an apparently narrow molecular receptive range, responding strongly to only one or two terpenoid odorants. The second subset was activated exclusively by aromatics and responded strongly to two to seven odorants. The third subset had a broad molecular receptive range and responded strongly to odorants belonging to several chemical classes. We also found receptor cells that did not respond to any of the odorants tested but were spontaneously active. Certain odorants elicited excitatory responses in some sensilla but inhibitory responses in others, and some receptor cells were strongly excited by certain odorants but inhibited by others. Impregnation of groups of receptor cells in type-A trichoid sensilla with rhodamine-dextran demonstrated that their axons project mainly to the large female glomeruli of the antennal lobe.