Growth and gene expression are predominantly controlled by distinct regions of the human IL-4 receptor.
Growth and gene expression are predominantly controlled by distinct regions of the human IL-4 receptor.
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生长和基因表达主要由人类 IL-4 受体的不同区域控制。
DOI：
10.1016/s1074-7613(00)80677-9
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发表时间：
1996
期刊：
影响因子：
32.4
通讯作者：
Paul,WE
中科院分区：
文献类型：
--
作者：
Ryan,JJ;McReynolds,LJ;Keegan,A;Wang,LH;Garfein,E;Rothman,P;Nelms,K;Paul,WE
IL-4可促进造血细胞增殖并表达包括CD23在内的一系列基因。我们检查了IL-4介导的生长和基因诱导是否同样受到控制，通过4PS磷酸化测量。对表达人IL-4R截短突变体的M12.4.1细胞的研究表明，557-657氨基酸之间的区域是基因完整表达所必需的，这与Stat6 DNA结合活性有关。该区域不是4PS磷酸化所必需的。在氨基酸557-657之间的酪氨酸到苯丙氨酸的突变表明，只要一个酪氨酸保持不突变，CD23就被完全诱导。当三种酪氨酸都发生突变时，受体就不能诱导CD23。结果表明，IL-4R的生长调控和基因表达主要受IL-4R不同区域调控。
IL-4 causes hematopoietic cells to proliferate and express a series of genes, including CD23. We examined whether IL-4-mediated growth, as measured by 4PS phosphorylation, and gene induction were similarly controlled. Studies of M12.4.1 cells expressing human IL-4R truncation mutants indicated that the region between amino acids 557–657 is necessary for full gene expression, which correlated with Stat6 DNA binding activity. This region was not required for 4PS phosphorylation. Tyrosine-to-phenylalanine mutations in the interval between amino acids 557–657 revealed that as long as one tyrosine remained unmutated, CD23 was fully induced. When all three tyrosines were mutated, the receptor was unable to induce CD23. The results indicate that growth regulation and gene expression are principally controlled by distinct regions of IL-4R.