Constructing the metabolic and regulatory pathways in germinating rice seeds through proteomic approach
Constructing the metabolic and regulatory pathways in germinating rice seeds through proteomic approach
复制标题
DOI：
10.1002/pmic.201000598
复制
发表时间：
2011-07-01
期刊：
影响因子：
3.4
通讯作者：
Yang, Pingfang
中科院分区：
文献类型：
--
作者：
He, Dongli;Han, Chao;Yang, Pingfang
从蛋白质组数据构建代谢和调控途径可以将大规模数据置于生物体整体生理方案中。它是预测代谢表型或调控方式的有效方法。本研究采用LCMS/MS蛋白质组鸟枪法，通过1-DE技术对水稻萌发种子进行了蛋白质组分析。共鉴定出673种蛋白质，可分为14个功能组。最大的一组与代谢有关。代谢蛋白质整合到不同的代谢途径，以显示种子萌发过程中储备动员和前体准备的方式。对这些调控蛋白的分析表明，氧化还原稳态和基因表达的调节在水稻种子萌发中也起着重要作用。虽然转录对种子萌发是不必要的，但它可以保证种子萌发的快速性和一致性。相反，萌发需要用储存的mRNA进行翻译。本研究将有助于我们进一步了解水稻种子萌发过程中的代谢方式、氧化还原稳态的调节以及基因表达。
Construction of metabolic and regulatory pathways from proteomic data can contextualize the large-scale data within the overall physiological scheme of an organism. It is an efficient way to predict metabolic phenotype or regulatory style. We did protein profiling in the germinating rice seeds through 1-DE via LC MS/MS proteomic shotgun strategy. In total, 673 proteins were identified, and could be sorted into 14 functional groups. The largest group was metabolism related. The metabolic proteins were integrated into different metabolic pathways to show the style of reserves mobilization and precursor preparation during the germination. Analysis of the regulatory proteins indicated that regulation of redox homeostasis and gene expression also play important roles for the rice seed germination. Although transcription is unnecessary for the germination, it could ensure the rapidity and uniformity of germination. On the contrary, translation with the stored mRNA is required for the germination. This study will help us to further understand the metabolic style, regulation of redox homeostasis, and gene expression during rice seed germination.