Closing the lid on DNA end-to-end stacking interactions
Closing the lid on DNA end-to-end stacking interactions
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DOI：
10.1063/1.2937402
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发表时间：
2008-06-02
影响因子：
4
通讯作者：
Pollack, Lois
中科院分区：
文献类型：
--
作者：
Li, Li;Pabit, Suzette A.;Pollack, Lois
最近的实验表明，短的DNA链通过端到端堆叠联系在一起。在这里，我们报告了具有修饰末端的dna之间的相互作用。DNA双链，长20 bp，在2、1或0端用短的T-4环盖住，并置于含有20 mM Mg2+的溶液中。仅在具有一个或多个未封盖末端的构念中观察到关联。通过测量最小散射角下小角x射线散射（SAXS）曲线的变化来表征DNA-DNA相互作用。根据SAXS数据计算第二个病毒系数。我们的研究结果证实，端到端堆叠在短链DNA-DNA相互作用中起着重要作用。(c) 2008年美国物理研究所。
Recent experiments suggest that short DNA strands associate by end-to-end stacking. Here, we report interactions between DNAs with modified ends. DNA duplexes, 20 bp long, were capped with short T-4 loops at 2, 1 or 0 ends, and were placed in solutions containing 20 mM Mg2+. Association was observed only in constructs with one or more uncapped ends. DNA-DNA interactions were characterized by measuring variations in small angle x-ray scattering (SAXS) curves at the lowest scattering angles. Second virial coefficients were computed from the SAXS data. Our results confirm that end-to-end stacking plays an important role in short strand DNA-DNA interactions. (c) 2008 American Institute of Physics.