Long-term functional maintenance of primary human hepatocytes in vitro
Long-term functional maintenance of primary human hepatocytes in vitro
复制标题
原代人肝细胞体外长期功能维持
DOI：
10.1126/science.aau7307
复制
发表时间：
2019-04-26
期刊：
影响因子：
56.9
通讯作者：
Deng, Hongkui
中科院分区：
文献类型：
--
作者：
Xiang, Chengang;Du, Yuanyuan;Deng, Hongkui
The maintenance of terminally differentiated cells, especially hepatocytes, in vitro has proven challenging. Here we demonstrated the long-term in vitro maintenance of primary human hepatocytes (PHHs) by modulating cell signaling pathways with a combination of five chemicals (5C). 5C-cultured PHHs showed global gene expression profiles and hepatocyte-specific functions resembling those of freshly isolated counterparts. Furthermore, these cells efficiently recapitulated the entire course of hepatitis B virus (HBV) infection over 4 weeks with the production of infectious viral particles and formation of HBV covalently closed circular DNA. Our study demonstrates that, with a chemical approach, functional maintenance of PHHs supports long-term HBV infection in vitro, providing an efficient platform for investigating HBV cell biology and antiviral drug screening.
