Clonal identification by microsatellite loci in sporadic flowering of a dwarf bamboo species, Sasa cernua
Clonal identification by microsatellite loci in sporadic flowering of a dwarf bamboo species, Sasa cernua
复制标题
DOI：
10.1007/s10265-009-0220-1
复制
发表时间：
2009-05-01
影响因子：
2.8
通讯作者：
Kawahara, Takayuki
中科院分区：
文献类型：
--
作者：
Kitamura, Keiko;Kawahara, Takayuki
矮生竹种是一次结实的。它们同时开花，几十年后死亡。在Sasa属的开花类型范围从零星到群居。为了确定矮秆竹的零星开花是否是固定的遗传，我们调查了8个微卫星（SSR）位点的无性系分布在一个零星开花补丁的Sasa cernua Makino，一个主要的矮秆竹种发现在中部北海道。2006年5月，在1600 m（2）斑块中，60.5%的活秆发生开花。我们建立了一个50 × 10米的研究地块在这个补丁，并指出1267丛2529活秆组成。我们调查了所有的1267丛，并确定了6个多位点基因型克隆使用5个可变的SSR位点。所有的开花丛都属于同一个克隆。另一方面，不开花的营养丛也被发现是同一个克隆。这些数据表明，所有的开花秆起源于一个单一的克隆的零星开花补丁的S。cernua。利用克隆分析法研究紫云英的零星开花。cernua透露，只有一部分克隆开花和死亡，而不是整个克隆。
Dwarf bamboo species are monocarpic. They flower simultaneously and die after several decades. The type of flowering in the genus Sasa ranges from sporadic to gregarious. In order to determine whether or not the sporadic flowering of dwarf bamboo is fixed genetically, we investigated the distribution of clones using eight microsatellite (SSR) loci in a sporadic flowering patch of Sasa cernua Makino, a major dwarf bamboo species found in central Hokkaido. In May 2006, flowering occurred on 60.5% of living culms in a 1600 m(2) patch. We established a 50 x 10 m study plot in this patch and noted 1267 clumps consisting of 2529 living culms. We investigated all 1267 clumps and identified six multilocus genotypes as clones using five variable SSR loci. All flowering clumps belonged to the same clone. On the other hand, non-flowering vegetative clumps were also discovered to be of the same clone. These data suggest that all flowering culms originated from a single clone of a sporadic flowering patch of S. cernua. Clonal analysis for investigation of sporadic flowering of S. cernua revealed that only a portion of a clone flowers and dies instead of the whole clone.