Localization of sphingosine kinase-1 in mouse sperm acrosomes
Localization of sphingosine kinase-1 in mouse sperm acrosomes
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DOI：
10.1369/jhc.4b6507.2005
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发表时间：
2005-02-01
影响因子：
3.2
通讯作者：
Nozawa, Y
中科院分区：
文献类型：
--
作者：
Matsumoto, K;Banno, Y;Nozawa, Y
鞘氨醇激酶(SPHK)催化鞘氨醇磷酸化，形成一种具有生物活性的脂质介体-1-磷酸鞘氨醇(S1P)。在目前的研究中，我们报告了SPHK-1在小鼠精子中的存在。SPHK-1定位于精子顶体，RT-PCR和Western印迹分析证实其表达。小鼠精子的SPHK活性为18.1pmol/min/mg蛋白。此外，我们还通过RT-PCR鉴定了S1P受体S1P(1)、S1P(2)、S1P(3)和S1P(5)在小鼠精子中的存在。这些结果提示SPHK-1产生的S1P可能通过S1P受体在顶体反应中发挥作用。
Sphingosine kinase (SPHK) catalyzes sphingosine phosphorylation to form a bioactive lipid mediator, sphingosine-1-phosphate (S1P). In the current study, we report the presence of SPHK-1 in mouse spermatozoa. SPHK-1 was localized to the acrosomes of spermatozoa, and its expression was proven by RT-PCR and Western blot analysis. SPHK activity of mouse spermatozoa was 18.1 pmol/min/mg protein. Furthermore, we identified the presence of the S1P receptors S1P(1), S1P(2), S1P(3), and S1P(5), in mouse spermatozoa by RTPCR. These results suggest that S1P produced by SPHK-1 would play a role in the acrosomal reaction through S1P receptors.