Calcium dynamics in neurons treated with toxic and non-toxic concentrations of glutamate.
Calcium dynamics in neurons treated with toxic and non-toxic concentrations of glutamate.
复制标题
用有毒和无毒浓度的谷氨酸处理的神经元中的钙动态。
作者：
M. DeCoster;M. Koenig;J. Hunter;F. Tortella
同时监测细胞内钙浓度（[Ca 2 +]i）动态在多个培养的大鼠神经元加载Fluo-3和连续刺激谷氨酸（GLU）。观察到三种反应类型：10 μ M GLU引起[Ca 2 +]i的初始短暂增加; 20 μ M为双相反应，其特征是峰值之间有150-350 s的“钙谷”; 40 μ M为[Ca 2 +]i的初始持续增加。在无钙培养基中用40 μ M GLU处理的神经元仅显示[Ca 2 +]i的初始瞬时增加，表明[Ca 2 +]i的持续继发性增加依赖于细胞外钙源。我们观察到一个给定的培养液内的多个神经元的同步反应，GLU治疗后，支持细胞外钙的持续流入的假设，细胞内钙的耗竭和/或内源性兴奋性氨基酸的释放可能会刺激。
Intracellular calcium concentration ([Ca2+]i) dynamics were simultaneously monitored in multiple cultured rat neurons loaded with Fluo-3 and continuously stimulated with glutamate (GLU). Three response types were observed: 10 microM GLU caused an initial transient increase in [Ca2+]i; 20 microM a biphasic response characterized by a 150-350 s 'calcium trough' between peaks; and 40 microM an initial sustained increase in [Ca2+]i. Neurons in calcium-free medium treated with 40 microM GLU showed only an initial transient increase in [Ca2+]i, demonstrating the dependence of sustained secondary increases in [Ca2+]i on extracellular calcium sources. We observed synchronized responses of multiple neurons within a given culture well, after GLU treatment, supporting the hypothesis that sustained influx of extracellular calcium may be stimulated by depletion of intracellular calcium and/or the release of endogenous excitatory amino acids.