REal-time Assessment of Community Transmission (REACT) of SARS-CoV-2 virus: Study protocol.
REal-time Assessment of Community Transmission (REACT) of SARS-CoV-2 virus: Study protocol.
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DOI：
10.12688/wellcomeopenres.16228.1
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发表时间：
2020-01-01
影响因子：
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通讯作者：
Elliott, Paul
中科院分区：
文献类型：
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作者：
Riley, Steven;Atchison, Christina;Elliott, Paul
背景：英国英格兰是全球确诊的 COVID-19 死亡率最高的国家之一。直到最近，SARS-CoV-2 病毒的检测主要集中在医疗保健和护理院环境中。因此，人们对社区传播的了解要少得多。方案：社区传播实时评估 (REACT) 计划是一项针对 COVID-19 进行家庭检测的主要计划，旨在跟踪社区感染的进展情况。 REACT-1 涉及对英格兰各地随机选择的 100,000 至 150,000 名个体的重复样本进行病毒检测（RT-PCR 病毒学拭子）测试的横断面调查。这检查了病毒传播的范围以及目前有多少人被感染。年龄范围为5岁及以上。个人是从英格兰 NHS 患者名单中抽取的。 REACT-2 是一系列五项子研究，旨在确定英国 SARS-CoV-2 抗体的血清阳性率作为历史感染的指标。主要研究（研究 5）采用与 REACT-1 相同的设计和采样方法，在 100,000 至 200,000 名 18 岁及以上成年人的重复样本中使用自我管理的侧流免疫分析 (LFIA) 测试 IgG 抗体。为了为研究 5 提供信息，研究 1-4 评估了 SARS-CoV-2 LFIA 的性能特征（研究 1）以及 LFIA 在不同人群中进行家庭检测的可行性、可用性和应用的不同方面（研究 2-4）。伦理和传播：该研究已获得伦理批准。结果使用 STROBE 指南进行报告，并通过向公共卫生机构提交的报告、科学会议上的演示和开放获取出版物进行传播。结论：这项研究提供了英格兰普通人群中病毒流行率（RT-PCR，REACT-1）和血清流行率（抗体，REACT-2）的可靠估计。我们还探索了 LFIA 在家庭环境中进行 SARS-CoV-2 抗体自我检测的可接受性和可用性，这在普通人群中以前从未进行过如此大规模的检测。
Background: England, UK has one of the highest rates of confirmed COVID-19 mortality globally. Until recently, testing for the SARS-CoV-2 virus focused mainly on healthcare and care home settings. As such, there is far less understanding of community transmission. Protocol: The REal-time Assessment of Community Transmission (REACT) programme is a major programme of home testing for COVID-19 to track progress of the infection in the community. REACT-1 involves cross-sectional surveys of viral detection (virological swab for RT-PCR) tests in repeated samples of 100,000 to 150,000 randomly selected individuals across England. This examines how widely the virus has spread and how many people are currently infected. The age range is 5 years and above. Individuals are sampled from the England NHS patient list. REACT-2 is a series of five sub-studies towards establishing the seroprevalence of antibodies to SARS-CoV-2 in England as an indicator of historical infection. The main study (study 5) uses the same design and sampling approach as REACT-1 using a self-administered lateral flow immunoassay (LFIA) test for IgG antibodies in repeated samples of 100,000 to 200,000 adults aged 18 years and above. To inform study 5, studies 1-4 evaluate performance characteristics of SARS-CoV-2 LFIAs (study 1) and different aspects of feasibility, usability and application of LFIAs for home-based testing in different populations (studies 2-4). Ethics and dissemination: The study has ethical approval. Results are reported using STROBE guidelines and disseminated through reports to public health bodies, presentations at scientific meetings and open access publications. Conclusions: This study provides robust estimates of the prevalence of both virus (RT-PCR, REACT-1) and seroprevalence (antibody, REACT-2) in the general population in England. We also explore acceptability and usability of LFIAs for self-administered testing for SARS-CoV-2 antibody in a home-based setting, not done before at such scale in the general population.