Inflammation and oxidative stress in end-stage renal disease patients treated with hemodialysis or peritoneal dialysis
Inflammation and oxidative stress in end-stage renal disease patients treated with hemodialysis or peritoneal dialysis
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DOI：
10.1177/039139880903201206
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发表时间：
2009-12-01
影响因子：
1.7
通讯作者：
Vlassopoulos, Dimosthenis
中科院分区：
文献类型：
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作者：
Filiopoulos, Vassilis;Hadjiyannakos, Dimitrios;Vlassopoulos, Dimosthenis
目的：不同透析方式对氧化应激和炎症的影响以及这种相互关系中涉及的因素尚未得到充分研究。本研究旨在比较血液透析（HD）和腹膜透析（PD）对氧化应激和炎症标志物的影响，并寻找相关因素。计算是基于总抗氧化能力（TAC）和超氧化物歧化酶（SOD），通过分光光度法，作为氧化应激生物标志物;和高敏C反应蛋白（hs-CRP），白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α），通过ELISA，作为炎症生物标志物。在TNF-α和IL-6中未观察到显著差异。与HD患者相比，PD患者显示TNF-α水平升高，尽管无显著性，但同型半胱氨酸（Hcy）显著升高。IL-6、hs-CRP、TAC和SOD无差异。在HD患者中，全段甲状旁腺激素（iPTH）和TNF-α之间以及尿酸（UA）和TAC之间存在显著正相关。β 2-微球蛋白（β 2 M）与TAC呈负相关，总胆固醇（TC）与TNF-α呈正相关，与SOD呈负相关，甘油三酯（TG）与TNF-α呈正相关。在PD患者中，TG与TNF-alpha正相关，HDL-胆固醇与TNF-alpha负相关，LDL-胆固醇与SOD负相关，β 2 M与SOD负相关。结论：HD和PD患者表现出相似程度的炎症和氧化应激激活。在HD和PD患者中，UA、iPTH、β 2 M和血脂等因素与氧化应激和炎症生物标志物相关。(Int J Artiff Organs 2009; 32：872-82）
Purpose: The impact of different dialysis modalities on oxidative stress and inflammation and the factors implicated in this interrelationship have not been adequately studied. This study was designed to comparatively evaluate the effect of hemodialysis (HD) and peritoneal dialysis (PD) on oxidative stress and inflammatory biomarkers and to search for associated factors.Methods: We studied 20 HD, 11 PD patients and 11 healthy controls. Calculations were based on total antioxidant capacity (TAC) and superoxide dismutase (SOD), by spectrophotometry, as oxidative stress biomarkers; and high sensitivity CRP (hs-CRP), Interleukin-6 (IL-6) and Tumor Necrosis Factor-alpha (TNF-alpha), by ELISA, as inflammation biomarkers.Results: HD and PD patients showed significantly increased levels of TAC, SOD and hs-CRP compared to healthy controls. No significant difference was observed in TNF-alpha and IL-6. Compared to HD patients, PD patients showed TNF-alpha levels that were increased, although non-significantly, and significantly higher homocysteine (Hcy). No differences were observed for IL-6, hs-CRP, TAC and SOD. In HD patients, significant positive correlations were found between intact parathyroid hormone (iPTH) and TNF-alpha, and between uric acid (UA) and TAC. beta 2-microglobulin (beta 2M) was negatively correlated with TAC, total cholesterol (TC) positively with TNF-alpha and negatively with SOD, and triglycerides (TG) correlated positively with TNF-alpha. In PD patients, TG correlated positively with TNF-alpha, HDL-cholesterol negatively with TNF-alpha, LDL-cholesterol negatively with SOD, and beta 2M negatively with SOD.Conclusions: HD and PD patients show similar degrees of inflammation and oxidative stress activation. Factors such as UA, iPTH, beta 2M and lipid profile correlate to oxidative stress and inflammatory biomarkers in both HD and PD patients. (Int J Artif Organs 2009; 32: 872-82)