Molecular epidemiology of African swine fever virus studied by analysis of four variable genome regions
Molecular epidemiology of African swine fever virus studied by analysis of four variable genome regions
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DOI：
10.1007/s00705-006-0794-z
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发表时间：
2006-12-01
影响因子：
2.7
通讯作者：
Dixon, L. K.
中科院分区：
文献类型：
--
作者：
Nix, R. J.;Gallardo, C.;Dixon, L. K.
非洲猪瘟病毒基因组的可变区包含串联重复序列的阵列，在40年期间获得的分离株的基因组中进行了比较。通过比较B602L和KP86R基因内四个不同基因组区域以及基因间隔区J286L和BtSj的聚合酶链式反应(PCR)产生的产物的大小，将来自欧洲、加勒比、西非和中非的43株密切相关的分离株归入17个不同的病毒亚组。对81个不同分离株的B602L基因中最可变的片段进行了序列分析，区分了31个亚群的病毒分离株，这些亚群的病毒分离株编码4个氨基酸的串联重复序列的序列和数量不同。因此，这些分析方法中的每一种都使以前通过对更保守的基因组区域进行测序而组合在一起的分离株能够被分成多个亚组。这提供了有关在不同国家流行的病毒株的更多信息。这些方法可在未来用于研究病毒分离株的流行病学和进化，并追踪疾病暴发的来源。
Variable regions of the African swine fever virus genome, which contain arrays of tandem repeats, were compared in the genomes of isolates obtained over a 40-year period. Comparison of the size of products generated by polymerase chain reaction (PCR) from four different genome regions, within the B602L and KP86R genes and intergenic regions J286L and BtSj, placed 43 closely related isolated from Europe, the Caribbean, West and Central Africa into 17 different virus sub-groups. Sequence analysis of the most variable fragment, within the B602L gene, from 81 different isolates distinguished 31 sub-groups of virus isolates which varied in sequence and number of a tandem repeat encoding 4 amino acids. Thus, each of these analysis methods enabled isolates, which were previously grouped together by sequencing of a more conserved genome region, to be separated into multiple sub-groups. This provided additional information about strains of viruses circulating in different countries. The methods could be used in future to study the epidemiology and evolution of virus isolates and to trace the sources of disease outbreaks.