A Plant Pathogen Type III Effector Protein Subverts Translational Regulation to Boost Host Polyamine Levels.
A Plant Pathogen Type III Effector Protein Subverts Translational Regulation to Boost Host Polyamine Levels.
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DOI：
10.1016/j.chom.2019.09.014
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发表时间：
2019-11
影响因子：
30.3
通讯作者：
Dousheng Wu;Edda von Roepenack-Lahaye;Matthias Buntru;Orlando de Lange;Niklas Schandry;Alvaro L Perez Quintero;Z. Weinberg;Tiffany M. Lowe-Power;B. Szurek;A. Michael;C. Allen;S. Schillberg;T. Lahaye
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文献类型：
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作者：
Dousheng Wu;Edda von Roepenack-Lahaye;Matthias Buntru;Orlando de Lange;Niklas Schandry;Alvaro L Perez Quintero;Z. Weinberg;Tiffany M. Lowe-Power;B. Szurek;A. Michael;C. Allen;S. Schillberg;T. Lahaye
病原菌将效应蛋白注入宿主细胞，操纵细胞过程，促进感染。转录激活子样效应子(TALES)是植物病原细菌中的一类效应器，通过转录激活寄主基因来促进疾病的发生。我们发现精氨酸脱羧酶(ADC)基因是植物青枯病菌Brg11的宿主靶标，Brg11是一种类似故事的效应器。Brg11针对的是一个17bp的序列，该序列是一个保守的50bp基序的一部分，称为ADC-box，位于参与多胺生物合成的ADC基因的上游。转录的ADC-box减弱了天然ADCmRNAs的翻译；然而，Brg11诱导了缺少ADC-box的截短的ADCmRNAs，从而绕过了这种翻译控制。因此，Brg11诱导多胺水平升高，从而触发防御反应，并可能抑制细菌利基竞争对手，但NOTR.Gsolanacearum。我们的发现表明，Brg11可能为青枯病提供了竞争优势，并揭示了细菌效应器在调节微生物-宿主-微生物三元相互作用中的作用。
Pathogenic bacteria inject effector proteins into host cells to manipulate cellular processes and facilitate the infection. Transcription-activator-like effectors (TALEs), an effector class in plant pathogenic bacteria, transcriptionally activate host genes to promote disease. We identifyarginine decarboxylase(ADC) genes as the host targets of Brg11, a TALE-like effector from the plant pathogenRalstonia solanacearum. Brg11 targets a 17-bp sequence that was found to be part of a conserved 50-bp motif, termed theADC-box, upstream ofADCgenes involved in polyamine biosynthesis. The transcribedADC-boxattenuates translation from nativeADCmRNAs; however, Brg11 induces truncatedADCmRNAs lacking theADC-box, thus bypassing this translational control. As a result, Brg11 induces elevated polyamine levels that trigger a defense reaction and likely inhibits bacterial niche competitors but notR. solanacearum. Our findings suggest that Brg11 may giveR. solanacearuma competitive advantage and uncover a role for bacterial effectors in regulating ternary microbe-host-microbe interactions.