Antioxidant activity of bisbenzylisoquinoline alkaloids from Stephania rotunda: cepharanthine and fangchinoline
Antioxidant activity of bisbenzylisoquinoline alkaloids from Stephania rotunda: cepharanthine and fangchinoline
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DOI：
10.3109/14756360902932792
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发表时间：
2010-02-01
影响因子：
5.6
通讯作者：
Topal, Fevzi
中科院分区：
文献类型：
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作者：
Gulcin, Ilhami;Elias, Riad;Topal, Fevzi
本研究采用体外抗氧化实验，包括1，1-二苯基-2-苦基-肼基（DPPH）自由基清除实验、2，2'-连氮双（azino-bis）自由基清除实验、2，2'-联氮双（azino-bis）自由基清除实验、2，2'-联氮双（azino-bis）自由基清除实验、2，2'-联氮双（azino-bis）自由基清除实验、2，2'-联氮双（azino-bis）自由基清除实验和2，2'-联氮双（azino-bis）自由基清除实验，（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基清除，N，N-二甲基-对苯二胺二盐酸盐（DMPD）自由基清除，超氧阴离子（O-2（中心点-））自由基清除、过氧化氢清除、总抗氧化活性、还原力和亚铁离子（Fe 2+）螯合活性。千金藤素和防己诺林碱在30 μ g/mL浓度下对亚油酸乳剂脂质过氧化反应的抑制率分别为94.6%和93.3%。另一方面，丁基羟基茴香醚（BHA），丁基羟基甲苯（BHT），α-生育酚，和trolox表明在相同浓度（30 μ g/mL）的亚油酸乳液的过氧化抑制83.3，92.2，72.4和81.3%，分别。结果表明，千金藤素和防己诺林碱具有较好的抗氧化和清除自由基的活性。
In the present study, we determined the antioxidant activity of cepharanthine and fangchinoline from Stephania rotunda by performing different in vitro antioxidant assays, including 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging, N,N-dimethyl-p-phenylenediamine dihydrochloride (DMPD) radical scavenging, superoxide anion (O-2(center dot-)) radical scavenging, hydrogen peroxide scavenging, total antioxidant activity, reducing power, and ferrous ion (Fe2+) chelating activities. Cepharanthine and fangchinoline showed 94.6 and 93.3% inhibition on lipid peroxidation of linoleic acid emulsion at 30 mu g/mL concentration, respectively. On the other hand, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), alpha-tocopherol, and trolox indicated inhibitions of 83.3, 92.2, 72.4, and 81.3% on peroxidation of linoleic acid emulsion at the same concentration (30 mu g/mL), respectively. According to the results, cepharanthine and fangchinoline have effective antioxidant and radical scavenging activity.