Age-related taste cell generation in circumvallate papillae organoids via regulation of multiple signaling pathways
Age-related taste cell generation in circumvallate papillae organoids via regulation of multiple signaling pathways
复制标题
通过调节多种信号通路在周乳头类器官中产生与年龄相关的味觉细胞
DOI：
10.1016/j.yexcr.2020.112150
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发表时间：
2020-09-15
影响因子：
3.7
通讯作者：
Yu, Yiqun
中科院分区：
文献类型：
--
作者：
Ren, Wenwen;Liu, Quan;Yu, Yiqun
在消化之前，味觉是评估食物的核心。味觉干细胞在一生中不断分化。然而，味觉受体细胞产生的机制尚不清楚。本研究通过Lgr5 +细胞和Lgr5细胞培养味觉类器官，发现环状乳头、叶状乳头和真菌状乳头中Lgr5 +细胞衍生的类器官更容易产生Car4 +和Gustducin +味觉受体细胞。新生小鼠环乳头Lgr5 +细胞衍生的味觉类器官比成年小鼠环乳头Lgr5 +细胞衍生的味觉类器官产生味觉受体细胞的能力更强。成年小鼠和新生小鼠的类器官在味觉转导等多种信号通路上存在巨大的转录差异。LY411575抑制Notch信号通路可增强环瓣乳突类器官味觉受体细胞的生成，调节多种信号通路。因此，我们得出结论，味觉类器官中受体细胞的产生与年龄有关，并通过多种信号通路进行调节。
Sense of taste is central to evaluate food before digestion. Taste stem cells undergo constant differentiation throughout the life. However, the mechanism underlying the generation of taste receptor cells is still not clear. Here, we cultured taste organoids from either Lgr5 + or Lgr5-cells, and found the preferential generation of Car4 + and Gustducin + taste receptor cells in organoids derived from Lgr5 + cells in circumvallate, foliate or fungiform papillae. Taste organoids derived from Lgr5 + cells in circumvallate papillae of neonatal mice showed stronger capacity to generate taste receptor cells compared to the organoids from Lgr5 + cells of the adult circumvallate papillae. Massive transcriptional differences were found in multiple signaling pathways including taste transduction between organoids derived from circumvallate papillae of adult and neonatal mice. Inhibiting the Notch signaling pathway by LY411575 enhanced taste receptor cell generation in organoids from circumvallate papillae and modulated multiple signaling pathways. Thus, we concluded that receptor cell generation in taste organoids was age-related and regulated via multiple signaling pathways.