Surface sulfonation and nitrification enhance the biological activity and osteogenesis of polyetheretherketone by forming an irregular nano-porous monolayer
Surface sulfonation and nitrification enhance the biological activity and osteogenesis of polyetheretherketone by forming an irregular nano-porous monolayer
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表面磺化和硝化通过形成不规则的纳米多孔单层增强聚醚醚酮的生物活性和成骨作用
DOI：
10.1007/s10856-019-6349-0
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发表时间：
2020-01-01
影响因子：
3.7
通讯作者：
Chen, Lei
中科院分区：
文献类型：
--
作者：
Li, Yanhua;Wang, Jing;Chen, Lei
聚醚醚酮（PEEK）正在成为临床脊柱和骨科应用的流行组分，但其实际应用受到一些限制。本研究通过磺化和硝化在聚醚醚酮表面形成了具有不同功能基团的不规则纳米多孔单分子层。通过场发射扫描电子显微镜、原子力显微镜、能量色散X射线光谱仪、水接触角测量仪和傅里叶变换红外光谱仪对表面特征进行了检测。通过细胞粘附、形态学变化、增殖、碱性、磷酸酶活性、实时荧光定量PCR和western blot分析来评价细胞的体外行为。通过显微CT和组织学评估检查体内骨整合。我们的研究结果表明，不规则的纳米孔PEEK的生物性能的影响。高温水热NP处理诱导早期成骨分化和早期成骨。通过磺化和硝化进行改性可以拓宽PEEK在骨科和牙科应用中的用途。本研究为PEEK的临床应用提供了理论依据。a为了获得均匀的多孔结构，采用浓硫酸和发烟硝酸（82-80%）对PEEK样品进行磁力搅拌处理。b纳米孔对bMSCS生物学行为的影响。
Polyether-ether-ketone (PEEK) is becoming a popular component of clinical spinal and orthopedic applications, but its practical use suffers from several limitations. In this study, irregular nano-porous monolayer with differently functional groups was formed on the surface of PEEK through sulfonation and nitrification. The surface characteristics were detected by field-emission scanning electron microscopy, atomic force microscopy, energy-dispersive X-ray spectrometry, water contact angle measurements and Fourier transform infrared spectroscopy. In vitro cellular behaviors were evaluated by cell adhesion, morphological changes, proliferation, alkalinity, phosphatase activity, real-time RT-PCR and western blot analyses. In vivo osseointegration was examined through micro-CT and histological assessments. Our results reveal that the irregular nano-porous of PEEK affect the biological properties. High-temperature hydrothermal NP treatment induced early osteogenic differentiation and early osteogenesis. Modification by sulfonation and nitrification can broaden the use of PEEK in orthopedic and dental applications. This study provides a theoretical basis for the wider clinical application of PEEK.aTo obtain a uniform porous structure, PEEK samples were treated by concentrated sulfuric acid and fuming nitric acid (82–80%) with magnetic stirring sequentially.bEffects of nanopores on biological behavior of bMSCS.