ADRENAL-MEDULLARY GANGLION NEURONS PROJECT INTO THE SPLANCHNIC NERVE
ADRENAL-MEDULLARY GANGLION NEURONS PROJECT INTO THE SPLANCHNIC NERVE
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DOI：
10.1016/0306-4522(95)00305-3
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发表时间：
1995-12-01
期刊：
影响因子：
3.3
通讯作者：
HOKFELT, T
中科院分区：
文献类型：
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作者：
DAGERLIND, A;PELTOHUIKKO, M;HOKFELT, T
用逆行追踪法研究肾上腺髓质神经节神经元的投射。在肾上腺神经节附近切断内脏神经，并在神经横断部位应用逆行示踪剂FluoroGold。肾上腺髓质神经节神经元组显示荧光金或固蓝诱导的荧光仅限于胞体。使用免疫组织化学大多数逆行标记的神经节神经元显示神经肽Y的免疫反应性。此外，内脏神经切断后，大多数神经节神经元表达甘丙肽和甘丙肽信息相关肽免疫反应性，而在对照肾上腺中未观察到。综上所述，本研究结果强烈表明，肾上腺髓质神经节神经元投射回内脏神经，可能是调节肾上腺节前神经支配的反馈系统。
Retrograde tract-tracing was used to study the projections of adrenal medullary ganglion neurons. The splanchnic nerve was cut close to the suprarenal ganglia and the retrograde tracer FluoroGold was applied at the site of nerve transection. Groups of adrenal medullary ganglion neurons exhibited FlurorGold- or Fast Blue-induced fluorescence restricted to the perikarya. Using immunohistochemistry most retrogradely labelled ganglion neurons showed immunoreactivity for neuropeptide Y. In addition, after splanchnicotomy most ganglion neurons expressed galanin and galanin message-associated peptide immunoreactivities which could not be observed in control adrenals.Taken together, the present results strongly indicate that adrenal medullary ganglion neurons project back into the splanchnic nerve perhaps representing feedback system modulating the preganglionic innervation of the adrenal gland.