Development of co-dominant amplified polymorphic sequence markers in rice that flank the Magnaporthe grisea resistance gene Pi7(t) in recombinant inbred line 29
Development of co-dominant amplified polymorphic sequence markers in rice that flank the Magnaporthe grisea resistance gene Pi7(t) in recombinant inbred line 29
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DOI：
10.1094/phyto.2004.94.3.302
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发表时间：
2004-03-01
期刊：
影响因子：
3.2
通讯作者：
Ronald, PC
中科院分区：
文献类型：
--
作者：
Campbell, MA;Chen, D;Ronald, PC
Pi7(t)，一种源自水稻品种 Moroberekan 的显性抗稻瘟病基因。赋予对真菌病原体稻瘟病菌的完全抵抗力。 Pi7(t) 先前通过重组自交系群体的限制性片段长度多态性 (RFLP) 作图定位在染色体 II 上。该群体的一个衍生物，重组自交系(RIL)29，被指定为Pi7(t)的代表系。在 RIL29 前创建分离的 F-2 群体以确定 Pi7(t) 的位置。新的测绘数据表明 Pi7(t) 的位置距原始位置 30 厘摩。 Pi7(t) 与先前定位的 Pil M. grisea 抗性基因具有共同的位置。 RIL29 携带的 DNA 并非源自用于在新分配的 Pi7(t) 基因座处创建 RIL 群体的任何亲本。 RFLP 分析已确定了可能的供体来源。
Pi7(t), a dominant blast resistance gene derived from the rice cultivar Moroberekan. confers complete resistance against the fungal pathogen Magnaporthe grisea. Pi7(t) previously was positioned on chromosome I I by restriction fragment length polymorphism (RFLP) mapping of a recombinant inbred line population. One derivative of this population, recombinant inbred line (RIL)29, was designated as the representative line for Pi7(t). A segregating F-2 population was created front RIL29 in order to determine the location of Pi7(t). The new mapping data indicate a position for Pi7(t) 30 centimorgans distal to the original location. Pi7(t) shares a common position with the previously mapped Pil M. grisea resistance gene. RIL29 carries DNA not derived from either parent used to create the RIL population at the newly assigned Pi7(t) locus. RFLP analysis has identified a possible donor source.