Protocol for Rapid Fluorescence In Situ Hybridization of Bacteria in Cryosections of Microarthropods
Protocol for Rapid Fluorescence In Situ Hybridization of Bacteria in Cryosections of Microarthropods
复制标题
微型节肢动物冷冻切片中细菌快速荧光原位杂交实验方案
DOI：
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发表时间：
2003
影响因子：
4.4
通讯作者：
C. Tebbe
中科院分区：
文献类型：
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作者：
T. Thimm;C. Tebbe
摘要：建立了一种小亚基rrna靶向荧光原位杂交和显微技术检测微型节肢动物细菌的方法。该方案是基于整个标本的冷冻切片。与更常用的石蜡包埋技术相比，该方法更快，并且减少了可能损坏微观材料的操作次数。该方法可用于研究假丝酵母菌（Folsomia candida, Collembola）的定植以及肠道和组织中的细菌检测。
ABSTRACT A protocol was developed to detect bacteria inhabiting microarthropods by means of small-subunit rRNA-targeted fluorescence in situ hybridization and microscopy. The protocol is based on cryosections of whole specimens. In contrast to more commonly applied paraffin-embedding techniques, the protocol is quicker and reduces the number of manipulations which might damage the microscopic material. The method allowed the study of the bacterial colonization of Folsomia candida (Collembola) and the detection of bacteria in both the gut and tissue.