A sequencer coming of age: De novo genome assembly using MinION reads.
A sequencer coming of age: De novo genome assembly using MinION reads.
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DOI：
10.12688/f1000research.12012.1
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发表时间：
2017-01-01
期刊：
影响因子：
--
通讯作者：
Risse, Judith
中科院分区：
文献类型：
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作者：
de Lannoy, Carlos;de Ridder, Dick;Risse, Judith
Nanopore technology provides a novel approach to DNA sequencing that yields long, label-free reads of constant quality. The first commercial implementation of this approach, the MinION, has shown promise in various sequencing applications. This review gives an up-to-date overview of the MinION's utility as a de novosequencing device. It is argued that the MinION may allow for portable and affordable de novosequencing of even complex genomes in the near future, despite the currently error-prone nature of its reads. Through continuous updates to the MinION hardware and the development of new assembly pipelines, both sequencing accuracy and assembly quality have already risen rapidly. However, this fast pace of development has also lead to a lack of oversight in the expanding landscape of analysis tools, as performance evaluations are outdated quickly. Now that the MinION is approaching a state of maturity, a thorough comparative benchmarking effort of de novoassembly pipelines may be at place. An earlier version of this article can be found on BioRxiv.
