A DROSOPHILA HOMOLOG OF THE YEAST ORIGIN RECOGNITION COMPLEX
A DROSOPHILA HOMOLOG OF THE YEAST ORIGIN RECOGNITION COMPLEX
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DOI：
10.1126/science.270.5242.1674
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发表时间：
1995-12-08
期刊：
影响因子：
56.9
通讯作者：
BOTCHAN, MR
中科院分区：
文献类型：
--
作者：
GOSSEN, M;PAK, DTS;BOTCHAN, MR
已鉴定来自果蝇的基因编码与酿酒酵母起源识别复合物 (ORC) 的 Orc2p 和 Orc5p 同源的蛋白质。果蝇 Orc2p 同源物 DmORC2 的丰度受发育调节，在胚胎发生的最早阶段达到最高，同时 DNA 复制率最高。胚胎核提取物的分级分离表明，DmORC2 存在于与五个附加多肽紧密相关的复合物中，很像酵母 ORC。这些研究将能够直接测试后生动物中基于起始子的复制模型。
Genes from Drosophila melanogaster have been identified that encode proteins homologous to Orc2p and Orc5p of the Saccharomyces cerevisiae origin recognition complex (ORC). The abundance of the Drosophila Orc2p homolog DmORC2 is developmentally regulated and is greatest during the earliest stages of embryogenesis, concomitant with the highest rate of DNA replication. Fractionation of embryo nuclear extracts revealed that DmORC2 is found in a tightly associated complex with five additional polypeptides, much like the yeast ORC. These studies will enable direct testing of the initiator-based model of replication in a metazoan.