Genetic complementation in the Xenopus oocyte: co‐expression of sea urchin histone and U7 RNAs restores 3′ processing of H3 pre‐mRNA in the oocyte.
Genetic complementation in the Xenopus oocyte: co‐expression of sea urchin histone and U7 RNAs restores 3′ processing of H3 pre‐mRNA in the oocyte.
复制标题
非洲爪蟾卵母细胞中的遗传互补：海胆组蛋白和 U7 RNA 的共表达可恢复卵母细胞中 H3 pre-mRNA 的 3-2 加工。
DOI：
10.1002/j.1460-2075.1986.tb04411.x
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
M. Birnstiel
中科院分区：
文献类型：
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作者：
K. Strub;M. Birnstiel
在非洲爪哇卵母细胞中有效地3‘处理海胆组蛋白H3前-mRNA依赖于海胆U7 RNA或U7 RNP的共同注射。我们证明U7RNP是(Sm型)U-SnRNP家族的真正成员，通过U7RNA和U7RNP与多克隆抗三甲基鸟苷和单抗Sm抗体的免疫沉淀来判断。在非洲爪哇U2启动子的驱动下，海胆U7 RNA可以在青蛙卵母细胞中通过RNA基因产生。这样合成的转录本具有所有已知的真实U7 RNA的特征。我们的实验进一步表明，U7RNA 5‘端附近的序列介导了Sm的免疫沉淀能力。海胆U7RNA基因和H3组蛋白基因序列在青蛙卵母细胞中的共表达补充了组蛋白Pre-mRNA 3‘成熟缺陷。这提供了无可辩驳的证据，表明先前发现的海胆U7RNA确实是组蛋白mRNAs 3‘加工的重要组成部分。
Efficient 3′ processing of sea urchin histone H3 pre‐mRNA in the Xenopus oocyte is dependent on co‐injection of sea urchin U7 RNA or U7 RNP. We show that U7 RNP is a genuine member of the (Sm‐type) U‐snRNP family as judged by immunoprecipitation of the U7 RNA and U7 RNPs with polyclonal anti‐trimethylguanosine and monoclonal anti‐Sm antibodies. Sea urchin U7 RNA can be produced in the frog oocyte from RNA genes driven by a Xenopus U2 promoter. The transcripts thus synthesized have all the known characteristics of authentic U7 RNA. Our experiments further suggest that sequences near the 5′ end of the U7 RNA mediate Sm‐immunoprecipitability. Co‐expression of sea urchin U7 RNA gene and H3 histone gene sequences in the frog oocyte complements faulty 3′ maturation of histone pre‐mRNA. This provides irrefutable evidence that the sea urchin U7 RNA identified earlier is indeed an essential component of the 3′ processing of histone mRNAs.