Fungal diversity in anoxic-sulfidic sediments in a mangrove soil
Fungal diversity in anoxic-sulfidic sediments in a mangrove soil
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DOI：
10.1016/j.funeco.2011.09.004
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发表时间：
2012-04-01
期刊：
影响因子：
2.9
通讯作者：
Record, Eric
中科院分区：
文献类型：
--
作者：
Arfi, Yonathan;Marchand, Cyril;Record, Eric
真菌是红树林生态过程中的重要角色，被认为在有机物分解途径中发挥作用，尽管事实上红树林的碳处理很大一部分发生在缺氧的大量沉积物中。然而，这些地层中的真菌多样性知之甚少。在这里，使用荧光钙白色染色样品的显微镜观察，我们检测到真菌菌丝的存在，表明真菌生长发生在这些缺氧和硫化物condition.To评估真菌的多样性，我们使用454焦磷酸测序的核核糖体内部转录间隔区1和2（ITS-1和ITS-2）。我们分析了每个ITS的大约5000个读段，对应于大约115个分子操作分类单位。最丰富的操作分类单位隶属于：一个与Sistotremastrum属（Trechisporales）有亲缘关系的分类单元，Dipodeliasuraliensis（Pleosporales），一个链格孢属物种（Pleosporales）和一个未知的Lecanoromycete。本研究表明，在缺氧红树林沉积物中存在丰富的真菌群落。它还提出了这些生物对环境条件的适应问题以及它们在有机物分解过程中所起的作用问题。(C)2011 Elsevier Ltd和英国真菌学会。All rights reserved.
Fungi are important actors in the ecological processes occurring in mangroves and are thought to play a role in organic matter decomposition pathways, despite the fact that a large fraction of the carbon processing in mangroves happens in the anoxic bulk sediment. The fungal diversity in these strata is, however, poorly known. Here, using microscopic observations of calcofluor white stained samples, we detected the presence of fungal hyphae indicating that fungal growth was occurring under these anoxic and sulfidic conditions.To assess the fungal diversity we used 454 pyrosequencing of the nuclear ribosomal internal transcribed spacer land 2 (ITS-1 and ITS-2). We analysed approximately 5 000 reads for each ITS, corresponding to around 115 molecular operational taxonomic units.The taxonomic analyses identified the Agaricomycetes as the dominant fungal class. The most abundant operational taxonomic units were affiliated to: a taxon with affinity to the genus Sistotremastrum (Trechisporales), Dipodascus australiensis (Saccharomycetales), an Alternaria species (Pleosporales) and an unknown Lecanoromycete. This study indicates the presence of rich fungal communities in anoxic mangroves sediments. It also raises the question of the adaptation of these organisms to the environmental conditions and of the roles they play in organic matter decomposition processes. (C) 2011 Elsevier Ltd and The British Mycological Society. All rights reserved.