Bioremediation of acid mine water using facultatively methylotrophic metal-tolerant sulfate-reducing bacteria
Bioremediation of acid mine water using facultatively methylotrophic metal-tolerant sulfate-reducing bacteria
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DOI：
10.1016/s0944-5013(97)80025-0
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发表时间：
1997-02-01
影响因子：
6.7
通讯作者：
Babel, W
中科院分区：
文献类型：
--
作者：
Hard, BC;Friedrich, S;Babel, W
提出了一种微生物法处理高硫酸盐、高金属的酸性铀矿水。硫酸盐还原剂适用于这种工艺。硫酸盐的厌氧还原导致H2S的形成，H2S导致pH值的增加和金属的沉淀。甲醇是一种廉价、易得的碳源和能源。从一个污水池中分离出6株硫酸盐还原菌，1株从铜矿中分离出。所有菌株均以甲醇为电子供体和碳源。其他电子供体包括乳酸盐、丙酮酸盐和醋酸盐。菌株形态不均匀，细胞大小不一，呈棒状或弯曲状。所有菌株革兰氏阴性。以salexigens Desulfovibrio DSM 2538、africanus DSM 2603和d.c inolicus DSM 3852为对照菌株。其中1株UFZ b378在pH 4.0下生长，D. salexigens在pH 4.5下生长，其余菌株均不低于pH 6.0。采用不同pH值下不同硫酸盐和金属浓度的培养基模拟矿井水的组成。
A microbial process is proposed for the decontamination of acid uranium mine water high in sulfates and metals. Sulfate reducers are suitable for such a process. Anaerobic reduction of sulfate results in the formation of H2S which leads to an increase in pH and the precipitation of the metals. As cheap an readily available carbon and energy source methanol was chosen. Six strains of sulfate-reducing bacteria were isolated from a waste water pond, one from a copper mine. All strains were found to utilize methanol as electron donor and carbon source. Other electron donors included lactate, pyruvate and acetate. The strains were not uniform in their morphology, cells were of different sizes and rod-shaped or curved. All strains stained gram-negative. Desulfovibrio salexigens DSM 2538, D. africanus DSM 2603 and D. carbinolicus DSM 3852 were used as reference strains. One of the isolates named UFZ B 378 was found to grow at pH 4.0, D. salexigens at pH 4.5, all other strains did not grow below pH 6.0. Culture media with varying sulfate and metal concentrations at different pH values were used to simulate the composition of the mine waters.