LncRNA SNHG3 promotes cell growth by sponging miR-196a-5p and indicates the poor survival in osteosarcoma
LncRNA SNHG3 promotes cell growth by sponging miR-196a-5p and indicates the poor survival in osteosarcoma
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DOI：
10.1177/2058738418820743
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发表时间：
2019-01-09
影响因子：
3.5
通讯作者：
Zhang, Yong
中科院分区：
文献类型：
--
作者：
Chen, Jun;Wu, Zhouyi;Zhang, Yong
长链非编码RNA（lncRNA）的异常表达与多种恶性肿瘤的发生密切相关，而lncRNA小核仁RNA宿主基因（SNHGs）在肿瘤的发生发展中起着重要作用。然而，SNHG 3促进骨肉瘤（OS）的机制仍然难以捉摸。使用TCGA（The Cancer Genome Atlas）数据集分析OS患者中SNHG 3表达与临床病理特征之间的关联。通过MTT和集落形成试验估计细胞活力和集落数。通过荧光素酶报告测定证实miR-196 a-5 p与SNHG 3或H 0XC 8的特异性结合。结果，与邻近的正常组织相比，SNHG 3在OS组织中的表达显著增加。SNHG 3的高表达与肿瘤大小相关，是OS患者生存不良的独立预后因素。SNHG 3的敲低抑制细胞活力和集落形成，但其过表达逆转了这些作用。SNHG 3被进一步鉴定为miR-196 a-5 p的海绵，抵消了SNHG 3在OS细胞中引起的促肿瘤作用。miR-196 a-5 p的表达与SNHG 3及OS患者的生存率呈负相关。总之，lncRNA SNHG 3通过海绵状吸收miR-196 a-5 p促进细胞生长，并表明OS患者的预后不良。
Abnormal expression of long noncoding RNAs (lncRNAs) is closely associated with the pathogenesis of multiple malignancies, and lncRNA small nucleolar RNA host genes (SNHGs) play critical roles in tumor progression. However, the mechanism by which SNHG3 contributes to osteosarcoma (OS) remains elusive. The association between SNHG3 expression and the clinicopathological characteristics in OS patients was analyzed using the TCGA (The Cancer Genome Atlas) dataset. Cell viability and colony number were estimated by MTT and colony formation assays. MiR-196a-5p-specific binding with SNHG3 or HOXC8 was confirmed by the luciferase report assay. As a result, the expression of SNHG3 was dramatically increased in OS tissue as compared with the adjacent normal tissues. High expression of SNHG3 was associated with tumor size and acted as an independent prognostic factor of poor survival in OS patients. Knockdown of SNHG3 inhibited cell viability and colony formation, but its overexpression reversed these effects. SNHG3 was further identified to act as a sponge of miR-196a-5p, which counteracted the tumor-promoting effects caused by SNHG3 in OS cells. The expression of miR-196a-5p had a negative correlation with SNHG3 and the poor survival in OS patients. In conclusion, lncRNA SNHG3 promoted cell growth by sponging miR-196a-5p and indicated a poor prognosis in OS patients.