Comparative tissue uptake and cellular deposition of three different plutonium chemical forms in rats
Comparative tissue uptake and cellular deposition of three different plutonium chemical forms in rats
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DOI：
10.1080/09553000400005486
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发表时间：
2004-09-01
影响因子：
2.6
通讯作者：
Le Gall, B
中科院分区：
文献类型：
--
作者：
Fouillit, M;Grillon, G;Le Gall, B
目的：评价钚（Pu）的化学形态对其在组织和肝细胞内分布的影响。材料和方法：通过静脉注射Pu柠檬酸盐、Pu硝酸盐或Pu植酸盐污染雄性Sprague-Dawley大鼠组。在注射后不同时间测定各组织中钚的含量。PU肝脏分布进行了分析，放射自显影和细胞separation.Results：生物动力学研究表明，柠檬酸钚和硝酸钚主要保留在注射后的第一个小时内的骨骼，而大部分的钚是在肝脏注射后的植酸钚。放射自显影显示，柠檬酸钚均匀分布在肝脏中，而硝酸钚积累成“热点”。植酸钚呈中间型分布。肝细胞分离揭示了两种细胞类型之间的吸收的差异，这取决于注射的钚的化学形式，并在污染后的时间。结论：观察到不同的钚行为的生物活性，保留和肝脏分布。柠檬酸盐、硝酸盐和肌醇六磷酸盐之间的巨大差异可能由系统和肝脏转运的差异来解释。
Purpose: To evaluate the influence of the chemical form of plutonium (Pu) on its distribution in tissues and within liver cells populations.Materials and methods: Groups of male Sprague-Dawley rats were contaminated by intravenous injection of either Pu citrate, Pu nitrate or Pu phytate. Pu content was determined in various tissues at different times after injection. Pu liver distribution was analysed by autoradiography and after cellular separation.Results: Biokinetic studies indicate that Pu citrate and Pu nitrate predominantly retained in the skeleton within the first hours after injection, whereas most of the Pu was in the liver after injection of Pu phytate. Autoradiographs showed that Pu citrate was homogeneously distributed in the liver, while Pu nitrate accumulated into 'hot points'. Pu phytate showed an intermediate distribution pattern. Hepatic cell separation revealed a difference of uptake between the two cell types depending on the chemical form of injected Pu, and on the time after contamination.Conclusions: Distinct Pu behaviour was observed for biokinetics, retention and liver distribution. The large differences noted between citrate, nitrate and phytate might be explained by differences in systemic and hepatic transport.