A combination of hybrid polydopamine-human keratinocyte growth factor nanoparticles and sodium hyaluronate for the efficient prevention of postoperative abdominal adhesion formation
A combination of hybrid polydopamine-human keratinocyte growth factor nanoparticles and sodium hyaluronate for the efficient prevention of postoperative abdominal adhesion formation
复制标题
混合聚多巴胺-人角质形成细胞生长因子纳米颗粒和透明质酸钠的组合，可有效预防术后腹部粘连的形成。
DOI：
10.1016/j.actbio.2021.10.015
复制
发表时间：
2022-01-02
影响因子：
9.7
通讯作者：
Li, Xuqi
中科院分区：
文献类型：
--
作者：
Wei, Guangbing;Wang, Zijun;Li, Xuqi
Postoperative abdominal adhesion (PAA) is one of the more universal complications of abdominal surgery with a frequent incidence. Currently available keratinocyte growth factor (KGF)-based glues for the pre-vention of adhesions remain a great bottleneck since their long-term biological activity in vivo is insuf-ficient. In this study, we fabricated hybrid polydopamine (PDA)-KGF nanoparticles (PDA-KGF NPs) by us-ing an in situ self-assembly and polymerization method. The physicochemical properties of the PDA-KGF nanoparticles were systematically characterized. The effect of preventing PAA in rats was evaluated by us-ing hybrid PDA-KGF NPs combined with hyaluronate (Ha). The expression levels of inflammatory factors and the degree of inflammatory cell infiltration in the injured peritoneum were evaluated by enzyme-linked immunosorbent assays and hematoxylin-eosin staining, respectively. The levels of phospho-Src ex -pression were revealed by Western blotting. The degree of fibrosis and the density of deposited collagen fibers were measured with real-time reverse-transcription polymerase chain reaction and picrosirius red staining. The results indicated that the PDA-KGF NPs combined with Ha greatly prevented the incidence of abdominal adhesion s and promoted the repair of mesothelial cells in injured peritoneum. More impor-tantly, the PDA-KGF NPs combined with Ha obviously reduced collagen deposition and fibrosis and inhib-ited the inflammatory response. Our results suggest that PDA-KGF NPs combined with Ha are promising barrier-like biomaterials for the effective prevention of postoperative tissue adhesion.
