Karyotyping in Melon (Cucumis melo L.) by Cross-Species Fosmid Fluorescence in situ Hybridization
Karyotyping in Melon (Cucumis melo L.) by Cross-Species Fosmid Fluorescence in situ Hybridization
复制标题
通过跨物种 Fosmid 荧光原位杂交对甜瓜 (Cucumis melo L.) 进行核型分析
DOI：
10.1159/000314343
复制
发表时间：
2010-01-01
影响因子：
1.7
通讯作者：
Jin, W.
中科院分区：
文献类型：
--
作者：
Liu, C.;Liu, J.;Jin, W.
染色体鉴定对于细胞遗传学研究至关重要，并将加速遗传变异和育种的研究，特别是对于那些序列信息相对较少的物种。到目前为止，还没有开发出可靠的细胞学标志来区分甜瓜的个体染色体。本研究利用FISH（荧光原位杂交）结合比较基因组信息，筛选出21个SSR标记锚定的黄瓜fosmids作为甜瓜染色体的染色体特异性细胞学标记。此外，借助甜瓜着丝粒卫星DNA重复序列CentM、45S rDNA和5S rDNA，对同一中期细胞进行3组多fosmid混合物的连续FISH，使我们能够同时鉴定甜瓜12条中期染色体中的每一条，并构建了标准化的甜瓜体细胞中期染色体核型。最后，我们比较了甜瓜和黄瓜染色体之间 21 个 FISH 定位的 fosmids 分布，这使得我们能够更好地了解塑造这两个物种的进化过程。我们的研究为甜瓜基因组的细胞学表征和葫芦科的比较基因组学提供了基础。
Chromosome identification is critical for cytogenetic research and will accelerate studies on genetic variation and breeding, especially for those species with relatively little sequence information. So far, no reliable cytological landmarks have been developed to distinguish individual chromosomes in melon. In this study, using FISH (fluorescence in situ hybridization) combined with comparative genome information, we selected 21 cucumber fosmids anchored by SSR markers as chromosome-specific cytological markers for melon chromosomes. Moreover, with the help of melon centromeric satellite DNA repeats CentM, 45S rDNA and 5S rDNA, sequential FISH with 3 sets of multi-fosmid cocktails were conducted on the same metaphase cell, which allowed us to simultaneously identify each of the 12 metaphase chromosomes of melon and a standardized melon karyotype of somatic metaphase chromosomes was constructed. Finally, we compared the distribution of 21 FISH-mapped fosmids between melon and cucumber chromosomes, which allows a better understanding of the evolutionary process shaping these 2 species. Our study provides a basis for cytological characterization of the melon genome and comparative genomics of Cucurbitaceae.