Argon-Helium Cryosurgery for Treatment of C6 Gliomas in Rats and its Effect on Cellular Immunity
Argon-Helium Cryosurgery for Treatment of C6 Gliomas in Rats and its Effect on Cellular Immunity
复制标题
氩氦冷冻治疗大鼠C6胶质瘤及其对细胞免疫的影响
DOI：
10.1177/153303461000900110
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发表时间：
2010-02-01
影响因子：
2.8
通讯作者：
Miao，Li
中科院分区：
文献类型：
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作者：
Li，Ming;Zhang，Shizhong;Miao，Li
氩氦冷冻手术在某些实体瘤中显示出令人鼓舞的治疗效果，但其在胶质瘤治疗中的应用仍缺乏文献记载。为了探讨胶质瘤病灶细胞凋亡及氩氦冷冻后细胞免疫功能的变化，我们建立Wistar大鼠皮下C6胶质瘤模型，将大鼠分为正常对照组（20只）、假手术组（32只）、手术切除组（20只）和冷冻组（32只），并进行相应的处理。观察术后磁共振成像（MRI）和肿瘤细胞形态学变化，TUNEL法观察肿瘤灶细胞凋亡情况。采用流式细胞术分析冷冻手术后T淋巴细胞亚群的变化。结果表明，低温手术不仅能引起肿瘤细胞的破坏，还能引起肿瘤病灶周围细胞的凋亡。与手术切除导致CD3+和CD4+细胞百分比显著降低相比，冷冻手术导致CD3+和CD4+细胞百分比显著升高（P<0.05），术后14天CD4+/CD8+细胞比例相对升高。这些结果表明，除了破坏肿瘤细胞外，冷冻手术还能增强C6胶质瘤大鼠的细胞免疫和抗肿瘤免疫反应，提示氩氦冷冻手术在胶质瘤的临床治疗中具有很大的潜力。
Argon-helium cryosurgery has shown encouraging therapeutic effects on some solid tumors, but its application in the treatment of gliomas remains poorly documented. To explore the cell apoptosis at the glioma foci and the cellullar immunity changes following argon-helium cryosurgery, we established Wistar rat models bearing subcutaneous C6 glioma and divided the rats into the normal control (20 rats), sham-operated (32 rats), surgical resection (20 rats), and cryosurgery (32 rats) groups with corresponding treatments. The postoperative changes in the findings by magnetic resonance imaging (MRI) and tumor cell morphology were observed, and the cell apoptosis at the tumor foci was assessed with TUNEL assay. Flow cytometry was performed for analysis of the T lymphocyte subset changes following the cryosurgery. The results showed that cryosurgery induced not only destruction of the tumor cells but also cell apoptosis around the cryablation foci. Compared with surgical resction that caused significant reduction in CD3+ and CD4+ cell percentages, cryosurgery resulted in significantly increased percentages of CD3+ and CD4+ cells (P<0.05) with a relative increase of the CD4+/CD8+ cell ratio 14 days after the operation. These results demonstrate that in addition to tumor cell destruction, cryosurgy also results in enhanced cellular immunity and antitumor immune response of the C6 glioma-bearing rats, suggesting the great potential of argon-helium cryosurgery in clinical management of gliomas.