Differential regulation of anterior pituitary corticotrope function is observed in vivo but not in vitro in two lines of ethanol-sensitive mice.
Differential regulation of anterior pituitary corticotrope function is observed in vivo but not in vitro in two lines of ethanol-sensitive mice.
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在两个对乙醇敏感的小鼠系中，在体内观察到垂体前叶皮质激素功能的差异调节，但在体外未观察到。
DOI：
10.1111/j.1530-0277.1990.tb00454.x
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Wand,GS
中科院分区：
文献类型：
--
作者：
Wand,GS
分析了长睡眠（LS）和短睡眠（SS）系小鼠的垂体前叶促肾上腺皮质激素功能，这些小鼠是根据对乙醇的敏感性差异而选择性饲养的。在体内的挑战与急性乙醇或CRH管理或压力的新的处理导致更明显的增加，血清皮质酮水平在LS小鼠相比，SS小鼠。同样，体内给予乙醇导致LS小鼠垂体前叶促肾上腺皮质激素原/内啡肽mRNA水平比SS小鼠高3倍。然而，在体外研究垂体前叶促肾上腺皮质激素功能时，在体内分析中未观察到HPA轴的这种差异调节。LS和SS前垂体细胞的原代培养物对已知刺激垂体前叶ACTH分泌的各种促分泌素的反应适当但相当。这些促分泌素包括CRH（10 nm）、二丁酰-cAMP（1 mm）、加压素（100 nm）和佛波醇12-肉豆蔻酸酯13-乙酸酯（10 nm）。乙醇对垂体ACTH分泌无直接刺激作用。通过[35 S]蛋氨酸标记的垂体前叶外植体和[35 S]蛋氨酸标记的垂体前叶细胞原代培养物提取物的免疫沉淀和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，测定垂体前叶促肾上腺皮质激素肽生物合成的定量。LS小鼠垂体外植体中促肾上腺皮质激素原/内啡肽的生物合成是SS小鼠垂体外植体中促肾上腺皮质激素原/内啡肽生物合成的2倍。然而，在从下丘脑和肾上腺因子中分离的培养物中，LS垂体前叶促肾上腺皮质激素原/内啡肽生物合成速率变得与SS垂体前叶促肾上腺皮质激素原/内啡肽生物合成速率相等。结果表明，在体内观察到的LS和SS垂体前叶促肾上腺皮质激素的差异性调节不是由于LS和SS垂体前叶促肾上腺皮质激素功能的遗传差异。
Anterior pituitary corticotrope function was analyzed in the long sleep (LS) and short sleep (SS) lines of mice selectively bred for differences in sensitivity to ethanol. In vivo challenge with acute ethanol or CRH administration or the stress of novel handling resulted in a more pronounced increase in serum corticosterone levels in LS mice compared with SS mice. Likewise, in vivo administration of ethanol resulted in 3‐fold higher levels of anterior pituitary pro‐ACTH/endorphin mRNA in LS mice compared with SS mice. However, this differential regulation of the HPA axis during in vivo analysis was not observed during in vitro studies of anterior pituitary corticotrope function. Primary cultures of LS and SS anterior pituicytes responded appropriately but equivalently to a variety of secretagogues known to stimulate anterior pituitary ACTH secretion. These secretagogues included CRH (10 nm), dibutyryl‐cAMP (1 mm), vasopressin (100 nm), and phorbol 12‐myristate 13‐acetate (10 nm). Ethanol had no direct stimulatory effect on pituitary ACTH secretion. Quantitation of anterior pituitary corticotrope peptide biosynthesis was determined by immunoprecipitation and sodium dodecyl sulfate‐polyacrylamide gel electrophoresis of extracts from [35S]methionine‐labeled anterior pituitary explants and from [35S]methionine‐labeled primary cultures of anterior pituitary cells. LS mice pro‐ACTH/endorphin biosynthesis in pituitary explants was 2‐fold greater than pro‐ACTH/endorphin biosynthesis in SS mice pituitary explants. However, in culture, isolated from hypothalamic and adrenal factors, the LS anterior pituitary pro‐ACTH/endorphin biosynthetic rate became eguivalent to the SS anterior pituitary pro‐ACTH/endorphin biosynthetic rate. The results suggest that the differential regulation of LS and SS anterior pituitary corticotropes observed in vivo is not due to genetic differences in LS and SS anterior pituitary corticotrope function.