LINC02738 Participates in the Development of Kidney Cancer Through the miR-20b/Sox4 Axis.
LINC02738 Participates in the Development of Kidney Cancer Through the miR-20b/Sox4 Axis.
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DOI：
10.2147/ott.s262046
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发表时间：
2020
影响因子：
4
通讯作者：
Liu YS
中科院分区：
文献类型：
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作者：
Han C;Xu B;Zhou L;Li L;Lu C;Yu GP;Liu YS
长非编码RNA(LncRNAs)可以影响肿瘤的发生。来自癌症基因组图谱(TCAG)的数据表明，LINC02783在肾细胞癌(RCC)中高表达，有望成为潜在的生物学靶点。我们进行了这项研究来验证这一点。我们进行这项研究是为了验证LINC02783在肾癌中高表达的观点，并有望成为潜在的生物学靶点。采用实时定量聚合酶链式反应(qRT-PCR)检测LINC02783在肾癌组织中的表达，CKK-8法和Transwell法检测肾癌细胞的活性和侵袭力，Western印迹法检测Sox-4的表达，双荧光素酶报告(DLR)法和RNA免疫沉淀(RIP)法分析LINC02783与miR-20b的相互作用，体内实验检测肿瘤形成。我们在肾癌患者中检测到LINC02783的高表达。LINC02783水平高的患者预后明显较差。在体外和体内，LINC02783的下调抑制了肾癌细胞的活性和侵袭力。DLR分析结果表明，LINC02783通过调节miR-20b增强了SOX-4的表达。LINC02783可作为miR-20b抑制SOX-4表达的海绵。LINC02783在肾癌组织中高表达，预后不良。LINC02783可通过miR-20b/SOX-4轴影响肾癌的发生发展，有望成为治疗肾癌的靶点。
Long non-coding RNAs (lncRNAs) can affect tumorigenesis. Data from The Cancer Genome Atlas (TCAG) suggest that LINC02783 is highly expressed in renal cell carcinoma (RCC) and is expected to be a potential biological target. We conducted this study to verify this. We conducted this study to verify the opinion that “LINC02783 is highly expressed in renal cell carcinoma (RCC) and is expected to be a potential biological target”. We employed quantitative real-time polymerase chain reaction (qRT-PCR) to test LINC02783 expression in RCC tissues, CKK-8 assay and transwell assay to assess the viability and invasion of RCC cells, Western blot to quantify Sox-4 expression, dual-luciferase reporter (DLR) assay and RNA immunoprecipitation (RIP) assay to analyze the interaction between LINC02783 and miR-20b, in vivo experiments to test tumor formation. We detected high LINC02783 expression in RCC patients. Patients with higher LINC02783 levels had a markedly poorer prognosis. In vitro and in vivo, the down-regulation of LINC02783 suppressed the viability and invasion of RCC cells. The DLR assay results revealed that LINC02783 enhanced Sox-4 expression by regulating miR-20b. LINC02783 can act as a sponge for miR-20b to inhibit Sox-4 expression. LINC02783 is highly expressed in RCC patients and indicates a poor prognosis. LINC02783 can affect the occurrence and progression of RCC through the miR-20b/Sox-4 axis, making it a promising target for the treatment of RCC.